2017—2020年南宁市青年学生HIV抗体检测结果分析  

Analysis of Anti-HIV test results among young students in Nanning City from 2017 to 2020

作　　者：黄秋芳[1] 秦剑秋[1] 潘海西[1] 梁雪坚[1] 成肖阳 栾苑[1] 李梅 HUANG Qiu-fang;QIN Jian-qiu;PAN Hai-xi;LIANG Xue-jian;CHENG Xiao-yang;LUAN Yuan;LI Mei(Microbiological Laboratory,Nanning Center for Disease Control and Prevention,Guangxi 530023,China)

机构地区：[1]南宁市疾病预防控制中心微生物检验科,广西530023

出　　处：《医学动物防制》2022年第9期912-914,共3页Journal of Medical Pest Control

基　　金：广西壮族自治区卫生和计划生育委员会自筹基金计划项目(Z20200899)。

摘　　要：目的 通过对2017—2020年南宁市青年学生HIV抗体筛查有反应样本的复核和确证检测结果进行回顾性分析,为制定青年学生艾滋病防控措施提供参考。方法 收集2017—2020年南宁市青年学生HIV抗体筛查有反应样本161份,采用两种ELISA法试剂进行复核试验,再对复核试验中1种和/或2种试剂有反应的样本使用免疫印迹法(Western blotting, WB)进行确证,使用SPSS 22.0软件对数据进行统计分析。结果 2017—2020年南宁市青年学生HIV感染待确定样本161份,确证阳性60份,阳性率为37.27%;阴性95份,阴性率为59.01%;不确定6份,不确定率为3.73%。不同年份青年学生确证阳性结果差异有统计学意义(χ^(2)=24.487,P<0.01);不同性别学生HIV-1确证阳性率比较,差异有统计学意义(χ^(2)=43.713,P<0.01);不同文化程度学生HIV-1确证阳性率比较,差异有统计学意义(χ^(2)=6.929,P<0.05);不同样本来源学生HIV-1确证阳性率比较,差异有统计学意义(χ^(2)=84.443,P<0.01);WB检测反应条带gp160、gp120型出现率最高,均为100.00%;其次为p24型,出现率为98.33%;p55型出现率最低,为30.00%。结论 针对南宁市青年学生HIV-1检测情况,应严格控制检测质量,加强针对学生的有效干预,为尽早发现感染者提供质量保证。Objective To provide reference for the development of AIDS prevention and control measures for young students by retrospectively analyzing the reviewed and confirmed testing results of reactive samples in HIV-1 antibody screening and among young students in Nanning from 2017 to 2020.Methods A total of 161 reactive samples in HIV-1 antibody testing of young students in Nanning from 2017 to 2020 were collected, two ELISA reagents were used for rechecking and testing, Western blotting(WB) was used for confirmation, and SPSS 22.0 software was used for statistical analysis on the data.Results In 161 young students in Nanning from 2017 to 2020 undergoing confirmations on HIV infections, there were 60 confirmed positive cases with a positive rate of 37.27%,95 confirmed negative cases with a negative rate of 59.01%,and 6 uncertain cases with an uncertainty rate of 3.73%;the difference in the distribution of positive results among young students at different years was statistically significant when compared(χ^(2)=24.487,P<0.01);the difference in the positive rate of HIV-1 among students in different genders was statistically significant when compared(χ^(2)=43.713,P<0.01);the difference in the positive rate of HIV-1 among young students with different education levels was statistically significant when compared(χ^(2)=6.929,P<0.05);the difference in the HIV-1 confirmed positive rate for students from different sample sources was statistically significant when compared(χ^(2)=84.443,P<0.01);WB detection reaction bands gp160 and gp120 were found to have the maximum occurrence rate, and that value of both such indexes hit 100.00%,respectively, followed by p24 with an occurrence rate of 98.33%,and the occurrence rate of p55 was the minimum, hitting 30.00%.Conclusion The quality of HIV-1 testing should be strictly controlled according to the HIV-1 testing situation of young students in Nanning, and the quality assurance should be provided for early detection on HIV-1 infection.

关 键 词：青年学生 HIV-1 复核 免疫印迹法 结果分析 

分 类 号：R512.91[医药卫生—内科学]