HPLC-DAD法同时测定防脱发类化妆品中6种抗雄激素药物  

Simultaneous Determination of 6 Anti-androgen Drugs in Anti-hair-loss Cosmetics by HPLC-DAD

作　　者：刘丹 马莹[1] 杨健 李泽夏琼 LIU Dan;MA Ying;YANG Jian;LI Ze-xia-qiong(Guizhou Institute for Food and Drug Control,Guizhou Guiyang 550004,China)

机构地区：[1]贵州省食品药品检验所,贵州贵阳550004

出　　处：《广州化工》2022年第17期80-83,共4页GuangZhou Chemical Industry

基　　金：2020年国家化妆品监督抽检工作计划项目(国药监妆[2019]52号)。

摘　　要：建立了同时测定防脱发类化妆品中氟他胺、非那雄胺、左炔诺孕酮、醋酸环丙孕酮、醋酸氯地孕酮和度他雄胺等6种抗雄激素药物含量的HPLC-DAD方法。实验采用C色谱柱,柱温30℃,流动相为甲醇-水,等度洗脱;检测波长225 nm、285 nm。结果表明6种药物均在2~100μg·mL^(-1)范围内呈良好的线性关系(r>0.9990);经膏霜、乳液、水3种基质测试,平均加样回收率为97.6%~99.8%,RSD为0.05%~0.97%。该方法简便、重复性好,可用于判断防脱发类化妆品中6种抗雄激素药物的非法添加情况。A HPLC-DAD method for the simultaneous determination of the content of 6 anti-androgen drugs,including flutamide,finasteride,levonorgestrel,cycloproperosterone acetate,chlormadinone acetate and dutasteride in anti-hair-loss cosmetics was established.Preparation was performed on a 30℃Ccolumn,with the mobile phase comprising of methanol-water in a isocratic elution manner,and the detection wavelengths were set at 225 nm and 285 nm.The results showed that 6 drugs had a good linear relationship in the range of 2~100μg·mL^(-1)(r>0.9990).Tested in cream,emulsion and water matrices,the average recovery rate was 97.6%~99.8%with RSDs of 0.05%~0.97%.The method was simple and repeatable,which could effectively judge the illegal addition of 6 anti-androgen drugs in anti-hair-loss cosmetics.

关 键 词：高效液相色谱-二极管阵列检测器 防脱发 化妆品 抗雄激素药物 

分 类 号：R917[医药卫生—药物分析学]