葛根素对肥胖相关基因NPY、POMC和NMU表达的影响  

Effects of puerarin on the expression of obesity related genes NPY,POMC and NMU

作　　者：江道合 江青东[2] JIANG Daohe;JIANG Qingdong(Henan Zhengyang County Animal Husbandry Technology Service Center,Zhengyang Henan 463600;Institute of Animal Product Quality and safety technology,Henan University of Animal Husbandy and Economy,Zhengzhou Henan 450046)

机构地区：[1]河南正阳县畜牧技术服务中心,河南正阳463600 [2]河南牧业经济学院畜产品质量安全技术研究院,河南郑州450046

出　　处：《现代牧业》2022年第3期5-9,共5页Modern Animal Husbandry

基　　金：河南省科技攻关项目(202102110252);河南牧业经济学院博士科研启动基金项目(906/M4030073)。

摘　　要：研究葛根素对肥胖相关基因NPY、POMC和NMU表达的影响。选取体重250 g左右的SD雄性大鼠40只,随机分为4组:正常对照组、模型对照组、低剂量葛根素组、高剂量葛根素组,试验期42天。荧光定量PCR检测下丘脑NMU2R基因表达量,半定量PCR检测下丘脑NMU、NPY及POMC基因表达量。高剂量葛根素组NMU2R基因表达量显著高于正常对照组和模型对照组(P<0.05),低剂量葛根素组的NMU2R基因表达量高于高剂量葛根素组(P<0.05)。NPY基因表达在葛根素的诱导下减弱(P<0.05);POMC和NMU基因表达在葛根素的诱导下增加(P<0.05)。试验结果说明,葛根素的减肥机制与NPY、POMC的表达调控存在一定关联性。The experiment was to study the effect of puerarin on the expression of obesity related genes NPY,POMC and NMU.Forty male SD rats weighing about 250 g were randomly divided into 4 groups:normal control group and model control group,low dose puerarin group,high dose puerarin group.The test period was 42 days.The expression of hypothalamus NMU2R was detected by fluorescence quantification PCR,and the expressions of NMU,NPY and POMC genes in hypothalamus were detected by semi quantitative PCR.The expression of NMU2R gene in high-dose puerarin group was significantly higher than that in normal control group and model control group(P<0.05),and that in low-dose puerarin group was higher than that in high-dose puerarin group(P<0.05).NPY gene expression showed a weakening trend induced by puerarin(P<0.05).The expressions of POMC and NMU gene increased under the induction of puerarin(P<0.05).The results showed that the weight loss mechanism of puerarin was related to the expression and regulation of NPY and POMC.

关 键 词：葛根素 肥胖 神经肽Y 前皮质素原 神经介素U 

分 类 号：S567[农业科学—中草药栽培]