基于HPLC指纹图谱对红茴香和八角果实不同部位中莽草酸含量比较分析  

Comparative Analysis of Shikimic Acid Content in Different Parts of Illicium Henryi and Illicium Verum Fruits Based on HPLC Fingerprint

作　　者：李霞[1] 黄东萍 王仕宝[3,4] 赵苗苗[1] 李崇勇 何志鹏 崔艳丽[6] LI Xia;HUANG Dong-ping;WANG Shi-bao;ZHAO Miao-miao;LI Chong-yong;HE Zhi-peng;CUI Yan-li(School of Agricultural and Forestry Technology and Bioengineering,Hanzhong Vocation and Technology College,Hanzhong 723000,China;Hanzhong Center for Food and Drug Control,Hanzhong 723000,China;School of Pharmacy,Hanzhong Vocation and Technology College,Hanzhong 723000,China;Institute of Pharmaceutical(Edible)Botany of Qinba Mountains,Hanzhong Vocation and Technology College,Hanzhong 723000,China;Medical College of Shaanxi Institute of International Trade&Commerce,Xi’an 712046,China;Department of Basic Courses Teaching,Hanzhong Vocational and Technical College,Hanzhong 723000,China)

机构地区：[1]汉中职业技术学院农林技术与生物工程学院,陕西汉中723000 [2]汉中食品药品检验检测中心,陕西汉中723000 [3]汉中职业技术学院药学院,陕西汉中723000 [4]汉中职业技术学院秦巴山区药(食)用植物研究所,陕西汉中723000 [5]陕西国际商贸学院医药学院,陕西西安712046 [6]汉中职业技术学院基础课教学部,陕西汉中723000

出　　处：《食品与药品》2022年第5期391-395,共5页Food and Drug

基　　金：陕西省教育厅2020年度科学研究计划(编号:一般专项20JK0496);2017年中医药公共卫生服务补助专项“全国中药资源普查项目”(编号:财社[2017]66号);2019年汉中青年科技创新团队课题—秦巴山区药用植物研究团队;汉中职业技术学院课题(编号:HZYKYZRZD201906)。

摘　　要：目的HPLC测定红茴香和八角果实不同部位中莽草酸含量,并基于指纹图谱进行比较分析和相似度评价。方法色谱柱:Hedera NH2柱(250 mm×4.6 mm,5μm);流动相:乙腈-水(用磷酸调pH值3.5)(40:60);流速:1 ml/min;检测波长:210 nm;进样量:20μl。基于方法学考察并优化试验条件,先考察红茴香样品粉碎粒度、提取溶剂和时间,之后对样品进行含量测定。另外,结合中药指纹图谱软件进行相似度评价。结果莽草酸在5.61~560.56μg/ml范围内与峰面积呈良好的线性关系;平均回收率为96.57%。检测15批样品莽草酸,其得率分别为:红茴香果实96.14~178.33 mg/g;红茴香叶7.03~15.39 mg/g;茎皮中5.45 mg/g;八角果实不同部位(带籽果实、果壳、籽和果柄)含量为41.36~92.73 mg/g。结论本法精密度高,重复性好,可用于红茴香和八角中莽草酸的含量测定。中药指纹图谱对莽草酸含量比较分析和相似度评价表明,不同来源样品或同一基原不同部位莽草酸含量差异较大。Objective To determine the content of shikimic acid in different parts of Illicium henryi and Illicium verum fruits by HPLC and to perform the comparative analysis and similarity evaluation based on the fingerprint.Methods A Hedera NH2 column(250 mm×4.6 mm,5μm)was used with acetonitrile-water(adjusted pH to 3.5 with phosphoric acid)(40:60),as mobile phase.The flow rate was 1 ml/min,the detection wavelength was 210 nm and the injection volume was 20μl.Based on the study of methodology and the optimized conditions,the particle size,extraction solvent and time of Illicium henryi powder were investigated in detail,then the content of the sample was determined.In addition,the similarity evaluation was carried out by the fingerprint software of traditional Chinese medicine.Results The results showed that there was a good linear relationship between shikimic acid injection amount and peak area in the range of 5.61-560.56μg/ml.The average recovery was 96.57%.Shikimic acid was detected in 15 batches of samples,and the yield was 96.14-178.33 mg/g in Illicium henryi fruits,7.03-15.39 mg/g in Illicium henryi leaf,5.45 mg/g in stem bark.In addition,the contents of shikimic acid different parts of Illicium verum fruits(seeded fruits,shells,seed and fruit stalk)were 41.36-92.73 mg/g.Conclusion The HPLC method established in this study has high precision and good repeatability,and can be used for the determination of shikimic acid in Illicium henryi and Illicium verum fruits.At the same time,the comparative analysis and similarity evaluation based on the fingerprint of traditional Chinese medicine indicates that the content of shikimic acid in the samples from different sources or different parts of the same origin is quite different.

关 键 词：高效液相色谱法 红茴香 莽草酸 含量 指纹图谱 

分 类 号：R927[医药卫生—药学]