HPLC同时测定槐榆止血合剂中4种黄酮苷类成分含量  

Simultaneous Determination of Four Flavonoid Glycosides in Huaiyu Zhixue Mixture by HPLC

作　　者：杨军辉 蒋赢 俞宾 陈国宝[1] 袁保[1] 黄和军[1] 徐艺铨 YANG Jun-hui;JIANG Ying;YU bin;CHEN Guo-bao;Yuan Bao;HUANG He-jun;XU Yi-quan(Jiangyin Hospital of Traditional Chinese Medicine,Jiangyin 214400,China)

机构地区：[1]江阴市中医院,江苏江阴214400

出　　处：《食品与药品》2022年第5期424-427,共4页Food and Drug

基　　金：南京中医药大学自然科学基金(编号:XZR2020076);江阴市中医院院内课题(编号:202145)。

摘　　要：目的建立同时测定槐榆止血合剂中4种黄酮苷类成分芦丁、柚皮苷、橙皮苷、新橙皮苷含量的高效液相色谱法。方法采用Sepax C_(18)色谱柱(4.6 mm×250 mm,5μm)分离;以乙腈-0.2%磷酸溶液为梯度洗脱流动相;流速1 ml/min;检测波长283 nm;柱温35℃。结果4种黄酮苷类成分在相应范围内线性关系良好(r=1.0000),平均加样回收率99.53%~102.92%,RSD为1.49%~2.42%。结论该方法可用于槐榆止血合剂质量控制。Objective To determine 4 flavonoid glycosides of rutin,naringin,hesperidin and neohesperidin in Huaiyu Zhixue Mixture in by HPLC.Methods A Sepax C_(18) column(4.6 mm×250 mm,5μm)was adopted.The mobile phase was acetonitrile-0.2%phosphoric acid solution with gradient elution at a flow rate of 1 ml/min.The detection wavelength was 283 nm and the column temperature was 35℃.Results Four flavonoid glycosides showed good linear relationships within their own ranges(r=1.0000).The average recoveries were 99.53%-102.92%with the RSD of 1.49%-2.42%.Conclusion The method can be used for the quality control of Huaiyu Zhixue Mixture.

关 键 词：槐榆止血合剂 黄酮苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]