高效液相色谱-二极管阵列检测器法测定复方丹参片中12种成分及掺伪鉴别  

Determination of 12 Index Components and Illegally Added Components in Compound Danshen Tablets by High Performance Liquid Chromatography-Diode Array Detector

作　　者：范可青 凌霞 FAN Ke-qing;LING Xia(Hubei Province Xiangyang Public Inspection and Testing Center,Xiangyang 441000,Hubei China)

机构地区：[1]湖北省襄阳市公共检验检测中心,湖北襄阳441000

出　　处：《分析测试技术与仪器》2022年第3期353-357,共5页Analysis and Testing Technology and Instruments

摘　　要：采用高效液相色谱-二极管阵列检测器法测定复方丹参片中12种成分并判定是否掺有三七茎叶.流动相为乙腈-0.1%磷酸水溶液(梯度洗脱),通过变换波长结合紫外光谱扫描定性的方式对丹参素钠、原儿茶酸、原儿茶醛、迷迭香酸、丹酚酸B、三七皂苷R_(1)、人参皂苷Rg_(1)、人参皂苷Re、人参皂苷Rb_(1)、人参皂苷Rb_(3)、丹参酮Ⅰ、丹参酮ⅡA进行分析.12种成分的线性关系良好(r≥0.9992),精密度(RSD<2.0%)、稳定性(RSD<2.0%)、回收率(96.1%~99.8%)均符合方法学要求,可用于复方丹参片的质量控制.An analysis method has been established for the determination of 12 components in compound Danshen tablets by high performance liquid chromatography-diode array detector,and it was determined whether there were illegally mixed Panax notoginseng leaves in compound Danshen tablets.The mobile phase was acetonitrile-0.1%aqueous phosphate solution(gradient elution).The sodium Danshensu,protocatechuic acid,protocatechualdehyde,rosmarinic acid,salvianolic acid B,notoginsenoside R_(1),ginsenoside Rg_(1),ginsenoside Re,ginsenoside Rb_(1),ginsenoside Rb_(3),tanshinone Ⅰ,tanshinone ⅡA were analyzed by changing the wavelengthes and UV spectral scanning qualitative method.The linear relationship of the 12 components was good(r≥0.9992),and the precision(RSD<2.0%),stability(RSD<2.0%)and recovery rate(96.1%~99.8%)all met the methodological requirements,which can be used for the quality control of compound Danshen tablets.

关 键 词：高效液相色谱-二极管阵列检测器 复方丹参片 三七茎叶非法掺伪 

分 类 号：O657.7[理学—分析化学]