基于镧系金属有机框架纳米酶构建电化学生物传感器用于多巴胺的灵敏检测  

Lanthanide metal-organic frameworks based nanozyme electrochemical sensor with high sensitivity for depamine detection

作　　者：韩璐 贾丽丛 郝紫羽 陈连鑫 王贝贝[3] 牛永哲 康凯 籍雪平 HAN Lu;JIA Li-cong;HAO Zi-yu;CHEN Lian-xin;WANG Bei-bei;NIU Yong-zhe;KANG Kai;JI Xue-ping(Department of Pathogenic Biology,Hebei Medicine University,Shijiazhuang 050017,China;School of Public Health,Hebei Medicine University,Shijiazhuang 050017,China;Experimental Center,Hebei Medicine University,Shijiazhuang 050017,China;School of Pharmacy,Hebei Medicine University,Shijiazhuang 050017,China)

机构地区：[1]河北医科大学基础医学院,河北石家庄050017 [2]河北医科大学公共卫生学院,河北石家庄050017 [3]河北医科大学教学实验中心,河北石家庄050017 [4]河北医科大学药学院,河北石家庄050017

出　　处：《化学研究与应用》2022年第9期2104-2113,共10页Chemical Research and Application

基　　金：国家自然科学基金青年项目(82103890)资助;河北省自然基金项目(B2020206001)资助。

摘　　要：将三种镧系元素Ce、Tb、Gd为中心原子在电化学还原的石墨烯上原位合成了的金属有机框架(Metal-Organic Frameworks,MOFs)作为纳米酶,在玻碳电极表面构建了电化学生物传感器,并用于多巴胺(DA)的灵敏检测。应用X射线衍射仪(XRD)、X射线光电子能谱仪(XPS)等方法对所制备的MOFs进行了表征,并用循环伏安法(CV)和差分脉冲伏安法(DPV)研究了不同M-MOF(M=Ce、Tb、Gd)纳米酶的性能。根据研究结果,基于Ce-MOF构建的电化学传感器对多巴胺(DA)的反应具有最高的灵敏度和选择性,在0.1~100μM的范围内,其灵敏度为0.611 mA mM^(-1),检出限为6.47×10^(-8) M(S/N=3)。同时,该传感器还展现了良好的稳定性能和抗干扰能力,并且成功应用在实际样品测定中。Three Lanthanides(Ce,Tb,Gd),as the center atoms,were used to in situ synthesize metal-organic Frameworks(MOFs)on electrochemically reduced graphene.The electrochemical biosensors were constructed on the surface of glassy carbon electrode and used for sensitive detection of dopamine(DA).The morphology and structure of M-MOF(M=Ce、Tb、Gd)were characterized by X-ray diffraction(XRD)and X-ray photoelectron spectroscopy(XPS),and the properties of different M-MOF(M=Ce,Tb,Gd)nanozymes were studied by cyclic voltammetry(CV)and differential pulse voltammetry(DPV).According to the results,Ce-MOF dropped metal-organic frameworks based nanozyme electrochemical sensor exhibited a high sensitivity and selectivity for dopamine(DA),with the sensitivity of 0.611 mA·mM^(-1 )in the range of 0.1~100μM and a lower detection limit of 6.47×10^(-8) M(S/N=3).In addition,the sensor showed satisfactory stability and has been successfully applied to the detection of DA in samples.

关 键 词：金属有机框架 纳米酶 多巴胺 电化学传感器 

分 类 号：O657.1[理学—分析化学]