联合接种植物生长益生菌提高杂交象草在铜污染土壤修复中的效率  

Co-inoculation of plant growth-promoting rhizobacteria enhance phytoremediation efficiency of hybrid Pennisetum in Cu contaminated soils

作　　者：苗益博 杨傲 邢成广 刘蔚秋[1] MIAO Yibo;YANG Ao;XING Chengguang;LIU Weiqiu(Guangdong Provincial Key Laboratory of Plant Resources/School of Ecology,Sun Yat-sen University,Guangzhou 510275,China)

机构地区：[1]广东省热带亚热带植物资源重点实验室/中山大学生态学院,广东广州510275

出　　处：《中山大学学报（自然科学版）（中英文）》2022年第5期50-61,共12页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金(31070470,31370490)。

摘　　要：植物生长益生菌(PGPR,plantgrowth-promoting rhizobacteria)能提高植物修复的效率,虽然有研究显示联合接种可进一步提高植物修复效率,但目前人们仍大多关注于单菌株的促生作用,对于联合接种的作用和机理的研究仍有待进一步深入。我们将1~3种具有固氮能力或产1-氨基环丙烷-1-羧酸脱氨酶能力的耐铜PGPR菌株接种于杂交象草(Pennisetum americanum×P.purpureum)的种子上,研究在不同铜含量及不同土壤条件下其对杂交象草种子萌发、幼苗生长及铜吸收的影响,探讨联合接种具有互补促生功能的菌株能否进一步提高以及如何提高植物修复的效率。结果显示,植物生长益生菌能有效促进种子萌发及幼苗生长,且在高铜的条件下促生效果更好。杂交象草对铜耐受性强,其植物体对铜元素由根向茎迁移的系数低于0.1,接种PGPR进一步降低迁移系数。联合接种进一步增强了PGPR对植物地下部分生长的促进作用和对迁移效率的抑制作用。我们的研究显示联合接种2~3种具有互补植物生长促进性状的PGPR菌株可提高杂交象草在植物修复中的效率,而杂交象草可作为铜尾矿植物修复的候选物种,具有较高的潜在应用价值。Plant growth-promoting rhizobacteria(PGPR)can promote the efficiency of phytoremediation,but most studies have concerned on single PGPR strain and knowledge of the effects of co-inoculating multiple strains is still lacking.This study explored whether,and if so how,consortia of PGPR with complementary traits can increase phytoremediation efficiency.Hybrid Pennisetum(Pennisetum americanum×P.purpureum)seeds were inoculated with 1-3 Cu tolerant PGPR strains with N_(2)-fixing or 1-aminocyclopropane-1-carboxylate deaminase-producing ability,and the treatments’effects on seed germination,seedling growth and Cu uptake were assessed in a range of conditions.Our results showed that the PGPR strains promoted both seed germination and seedling growth,and the effects were stronger under the high-Cu conditions.Hybrid Pennisetum is quite tolerant to Cu and its root-to shoot translocation factor of Cu is lower than 0.1.PGPR inoculation further decreased the translocation factor.Co-inoculation of multiple strains enhanced both the stimulation effects on plant growth and the inhibition effects on translocation factor.Our results suggest that co-inoculation of 2-3 PGPR strains with complementary plant growth promoting traits can enhance the phytoremediation efficiency of hybrid Pennisetum,and hybrid Pennisetum is an excellent candidate plant for phytostabilization of Cu mine tailings.

关 键 词：重金属 尾矿 微生物辅助植物修复 联合接种 杂交象草(Pennisetumamericanum×P.purpureum) 

分 类 号：Q89[生物学] X753[环境科学与工程—环境工程]