鞍带石斑鱼Epinephelus lanceolatus卵子的短期保存  

Short-term storage of eggs from giant grouper(Epinephelus lanceolatus)

作　　者：魏千皓 叶智锋 王崇伟 林浩然[1] 李水生[1] 张勇[1] WEI Qianhao;YE Zhifeng;WANG Chongwei;LIN Haoran;LI Shuisheng;ZHANG Yong(State Key Laboratory for Biocontrol and Institute of Entomology/Guangdong Provincial Key Laboratory for Aquatic Economic Animals/School of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China)

机构地区：[1]有害生物控制与资源利用国家重点实验室/广东省水生经济动物良种繁育重点实验室/中山大学生命科学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）（中英文）》2022年第5期62-67,共6页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：2020年省级乡村振兴战略专项资金(渔业产业发展)(粤财农[2020]4);湛江湾实验室重大科研专项(ZJW-2019-06)。

摘　　要：在水产养殖中,建立体外保存卵子的方法对于人工授精和种苗生产具有重要的应用价值。鞍带石斑鱼Epinephelus lanceolatus是我国重要的水产养殖鱼类,其受精卵价格昂贵。本研究探讨了保存条件(保存介质、温度和pH)与添加剂(鞍带石斑鱼血清、维生素预混料)对于鞍带石斑鱼卵子短期保存的影响。结果表明,22℃时,卵子在L-15培养基(pH=8.1)中保存2 h,受精率和孵化率分别为66.4%和47.9%。在此基础上,添加鞍带石斑鱼血清和维生素预混料可以有效地延长保存时间,卵子能维持较高的活力。在体外保存8 h后,卵子的受精率和孵化率仍可达到64.4%和44.8%。本研究通过优化改良,建立了一种适合于鞍带石斑鱼卵子体外短期保存的体系,有助于鞍带石斑鱼的育种工作和孵化管理。Egg storage for fertilization and seed production has great significance in aquaculture.Giant grouper(Epinephelus lanceolatus)is an economically important aquaculture species in China,and its fertilized eggs are much expensive.In this study,we tried to develop an in vitro egg storage system in this species.Storage conditions such as medium,temperature and pH were experimentally investigated.Results showed that eggs stored in L-15 medium(pH=8.1)at 22℃for 2 h showed a relatively high fertilization rate(66.4%)and a hatching rate(47.9%).With supplementation of giant grouper serum and vitamin premix into L-15 medium,the storage time was prolonged and eggs still retained a relatively high viability.The fertilization and hatching rates from eggs stored for 8 h were 64.4%and 44.8%,respectively.This optimized short-term egg storage system would facilitate breeding practice and hatchery management in giant grouper.

关 键 词：鞍带石斑鱼Epinephelus lanceolatus 卵子保存 保存条件 血清 维生素 

分 类 号：Q492[生物学—生理学]