流式细胞术在遗传毒性评价中的应用  

Application of Flow Cytometry in Genotoxicity Evaluation

作　　者：唐茵茹 姜华[1] 王雪[1] 汪祺[1] 文海若 Tang Yinru;Jiang Hua;Wang Xue;Wang Qi;Wen Hairuo(National Institutes for Food and Drug Control,Beijing 100176,China;School of Pharmaceutical Sciences,Sun Yat-sen University,Guangzhou 510006,China)

机构地区：[1]中国食品药品检定研究院,北京100176 [2]中山大学药学院,广州510006

出　　处：《中国药事》2022年第9期1071-1077,共7页Chinese Pharmaceutical Affairs

基　　金：国家“重大新药创制”科技重大专项(编号2018ZX09201017);国家自然科学基金(编号81503347)。

摘　　要：遗传毒性是药物临床前安全性评价的重要组成部分,随着新型化合物数量的不断增加,建立高通量筛选方法以评价遗传毒性风险是当前药物研发人员的迫切需求。流式细胞术是一种能在短时间内对细胞进行多参数检测和分选的技术,具有自动化、高通量、特异性强等特点,目前已被应用于多种遗传毒性评价方法中。本文综述了流式细胞术在遗传毒性研究中的应用,并对其在遗传毒性评价上的应用前景进行探讨。Genotoxicity is an important part of the preclinical safety evaluation of drugs. With the growing numbers of new compounds, the establishment of high-throughput screening methods to evaluate genotoxicity is an urgent need for drug developers. Flow cytometry is a technology that can perform multi-parameter detection and cell sorting in a short period of time. It has the advantages of automation, high throughput and strong specificity. It has been used in a variety of genotoxicity evaluation. In this paper, the application of flow cytometry in the study of genotoxicity is reviewed, and its application prospect in the evaluation of genotoxicity is discussed.

关 键 词：流式细胞术 遗传毒性 高通量筛选 微核试验 Pig-a基因突变试验 γH2AX试验 

分 类 号：R95[医药卫生—药学]