电针对糖尿病肥胖大鼠下丘脑SOCS3、IRS-1蛋白表达及胰岛形态的影响  

Effect of electroacupuncture on protein expressions of SOCS3 and IRS-1 in hypothalamus and pancreas islet morphology in diabetic fatty rats

作　　者：庄舒婷 李瑞 宋姗姗 段浩茹 李秋艳 ZHUANG Shu-ting;LI Rui;SONG Shan-shan;DUAN Hao-ru;LI Qiu-yan(School of Acupuncture-Moxibustion and Tuina,Bejing University of CM,Beijing 100029,China)

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《中国针灸》2022年第9期1024-1028,共5页Chinese Acupuncture & Moxibustion

基　　金：国家自然科学基金面上项目:81973935。

摘　　要：目的:观察电针对Zucker糖尿病肥胖(ZDF)大鼠下丘脑细胞因子信号转导抑制蛋白3(SOCS3)、胰岛素受体底物蛋白-1(IRS-1)表达及胰岛形态的影响,探讨电针改善2型糖尿病(T2DM)血糖及胰岛素抵抗的可能机制。方法:选取SPF级雄性ZDF大鼠12只,以高脂饲料饲养4周诱导T2DM模型,造模成功后将其随机分为模型组和电针组,各6只;另取SPF级雄性Zucker瘦型大鼠6只作为空白组。电针组大鼠于双侧“胃脘下俞”“脾俞”“足三里”“三阴交”行电针干预,连续波,频率15 Hz,电流强度2 mA,每日1次,每次20 min,每周干预6次,共4周。于干预前后检测各组大鼠空腹血糖(FPG)。采用放射免疫法检测各组大鼠血清空腹胰岛素(FINS)水平,计算胰岛素抵抗指数(HOMA-IR);HE染色法观察各组大鼠胰岛形态学变化;Western blot法检测各组大鼠下丘脑SOCS3、IRS-1蛋白表达。结果:干预前,与空白组比较,模型组及电针组大鼠FPG升高(P<0.01)。干预后,与空白组比较,模型组大鼠FPG、血清FINS)水平及HOMA-IR升高(P<0.01),下丘脑SOCS3表达升高、IRS-1表达降低(P<0.01);与模型组比较,电针组大鼠FPG、血清FINS)水平及HOMA-IR降低(P<0.01),下丘脑SOCS3表达降低、IRS-1表达升高(P<0.01)。模型组大鼠胰岛形态不规则,胰岛面积、胰岛β细胞核数量减少,胰岛β细胞核代偿性增大;电针组胰岛形态、面积及胰岛β细胞核数量较模型组改善,胰岛β细胞核代偿性增大得到缓解。结论:电针可降低ZDF大鼠空腹血糖水平,改善胰岛形态,缓解胰岛素抵抗,其机制可能与下调下丘脑SOCS3蛋白表达、上调IRS-1蛋白表达,改善下丘脑调控外周糖代谢的功能有关。Objective To observe the effect of electroacupuncture(EA) on protein expressions of suppressor of cytokine signaling 3(SOCS3) and insulin receptor substrate-1(IRS-1) in hypothalamus and morphology of pancreas islet in Zucker diabetic fatty(ZDF) rats,and to explore its possible mechanism on improving plasma glucose and insulin resistance of type 2diabetes mellitus(T2DM).Methods Twelve SPF male ZDF rats were selected and fed with high-fat diet for 4 weeks to establish the T2DM model,after modeling,the rats were randomly divided into a model group and an EA group,6 rats in each one.Besides,6 SPF male Zucker lean rats were selected as a blank group.In the EA group,EA was applied at "Pishu"(BL 20),"Weiwanxiashu"(EX-B 3),"Zusanli"(ST 36) and "Sanyinjiao"(SP 6),with continuous wave,15 Hz in frequency,2 mA in intensity,once a day,20 min each time,6 times a week for 4 weeks.The fasting plasma glucose(FPG) was measured before and after intervention.The serum level of fasting insulin(FINS) was measured by radioimmunoassay,and the homeostasis model assessment of insulin resistance index(HOMA-IR) was calculated;the morphological change of pancreas islets was observed by HE staining;the protein expressions of SOCS3 and IRS-1 in hypothalamus were detected by Western blot.Results Before intervention,compared with the blank group,FPG in the model group and the EA group was increased(P<0.01).After intervention,compared with the blank group,FPG,serum level of FINS and HOMA-IR were increased(P<0.01),the protein expression of SOCS3 was increased while IRS-1 was decreased in the hypothalamus in the model group(P<0.01).Compared with the model group,FPG,serum level of FINS and HOMA-IR were decreased(P<0.01),the protein expression of SOCS3 was decreased while IRS-1 was increased in the hypothalamus in the EA group(P<0.01).In the model group,the shape of pancreas islets was irregular,the area of pancreas islets and the number of islet β cell nuclei were decreased,the nuclei of islet β cell was compensatory enlargement.In the EA group,th

关 键 词：2型糖尿病 电针 胰岛素抵抗 细胞因子信号转导抑制蛋白3 胰岛素受体底物蛋白-1 

分 类 号：R245.97[医药卫生—针灸推拿学]