肺炎克雷伯氏菌利用甘油生产1,3-PDO发酵优化的研究进展  

Advances in Optimizing Fermentation Conditions to Improve the Production of 1,3-Propanediol by Klebsiella pneumoniae Using Glycerol as Substrate

作　　者：王世伟[1] 王卿惠 向文胜[2] 李婷婷[1] 赵晨晨 周智强 WANG Shi-wei;WANG Qing-hui;XIANG Wen-sheng;LI Ting-ting;ZHAO Chen-chen;ZHOU Zhi-qiang(Key Lab.of Resist.Gene Engin.&Preserv.of Biodiver.in Cold Areas,Coll.of Life Sci.&Agric.&Forest.,Qiqihar Uni.,Qiqihar 161005;Schl.of Life Sci.,NE Agric.Uni.,Harbin 150030)

机构地区：[1]齐齐哈尔大学生命与农林学院抗性基因工程与寒地生物多样性保护重点实验室,黑龙江齐齐哈尔161005 [2]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《微生物学杂志》2022年第4期99-108,共10页Journal of Microbiology

基　　金：2021年度黑龙江省大学生创新创业训练计划资助项目(202110232101);2018年度黑龙江省省属本科高校基本科研业务费科研项目(135309482)。

摘　　要：发展可再生能源,尤其是生物能源,具有显著的能量收益和碳减排效益。随着石油等不可再生资源的减少,许多大宗传统石油化工产品正不断被使用可再生原料的生物制造产品替代。生物发酵法生产1,3-丙二醇(1,3-PDO)顺应了这一潮流,具有广阔的发展前景。提高微生物发酵竞争力,优化发酵法生产1,3-PDO水平,势必增加1,3-PDO的生产效益。对肺炎克雷伯氏菌(Klebsiella pneumoniae)发酵法进行1,3-PDO生产的代谢机理、菌株筛选和利用、发酵参数的选择和优化以及发酵工程策略的设计和监测等进行综述,为利用生物柴油副产物甘油生产有重要工业价值的1,3-PDO产品提供参考。The development of renewable energy,especially bioenergy,has significant energy and carbon emission reduction benefits.With the reduction of non renewable resources such as petroleum,many staple traditional petrochemical products are constantly replaced by industrial biological manufacturing products from renewable raw materials.Bio-fermentation production of 1,3-propanediol(1,3-PDO)conforms to this trend and has broad development prospects.Improving the competitiveness of microbial fermentation,optimizing the fermentation production level of 1,3-PDO and developing high-efficiency fermentation process are bound to increase the production benefits of 1,3-PDO.In this paper the metabolic mechanism,strain screening and utilization,selection and optimization of fermentation parameters,Klebsiella pneumoniae fermentation engineering strategy,as well as design,test and determination to produce 1,3-PDO are reviewed,so as to provide scientific references for the utilization of biodiesel byproduct production of glycerol to produce product of 1,3-PDO with significantly industrial value.

关 键 词：肺炎克雷伯氏菌 1 3-丙二醇 厌氧代谢机理 发酵 策略 

分 类 号：Q939.97[生物学—微生物学]