X射线辐照对淋巴细胞的灭活能力及对单采血小板质量的影响研究  

Effect of X-ray irradiation on the inactivation of lymphocytes and the quality of apheresis platelets

作　　者：程晨 陈怡静 张怡 卓海龙 骆群 CHENG Chen;CHEN Yijing;ZHANG Yi;ZHUO Hailong;LUO Qun(The Fifth Medical Center of PLA General Hospital,Beijing 100071,China)

机构地区：[1]解放军总医院第五医学中心,北京100071

出　　处：《中国输血杂志》2022年第8期821-824,共4页Chinese Journal of Blood Transfusion

摘　　要：目的探讨25Gy X射线辐照单采血小板后对其淋巴细胞的灭活能力及对单采血小板质量的影响。方法选取本中心2021年1~5月的健康无偿献血者20名,每人捐献单采血小板2袋,分为2组,分别用25Gy的X、γ射线辐照10 min;将上述辐照前后的单采血小板标本经淋巴细胞分离液分离出淋巴细胞,培养后分析比较X射线、γ射线辐照灭活单采血小板内淋巴细胞的能力;检测单采血小板辐照前及辐照后1、3 d的CD41b、CD62p、血常规、pH值。应用SPSS统计软件进行独立样本t检验分析比较组间差异。结果25Gy X射线、γ射线辐照后对淋巴细胞的抑制率分别为(98.034±1.778)%与(97.882±1.915)%。25Gy X射线辐照单采血小板后,与γ射线辐照组相比,1、3 d的Plt、PDW、MPV、P-LCR、PCT、pH、CD41b和CD62p值比较,差异均无统计学意义(P均>0.05)。结论25Gy X射线辐照能有效灭活单采血小板内的淋巴细胞,其灭活效果与γ射线相当,对淋巴细胞的增殖抑制率均能达到95%以上,且对单采血小板的质量没有显著影响。Objective To investigate the inactivation function of 25Gy X-ray irradiation on apheresis platelets’lymphocytes and its effect on the quantity of apheresis platelets(AP).Methods Twenty healthy voluntary AP donors from January to May 2021 in our center were selected,and 2 bags of AP were donated by each of them.The APs were divided into two groups to undergo X-ray andγ-ray irradiation for 10 min.Lymphocytes were separated from AP samples,before and after irradiation,by lymphocyte separation solution to analyze and compare the effect of X-ray andγ-ray on lymphocyte proliferation.The CD41b,CD62p,blood routine and pH of APs before and 1-day/3-day after irradiation were detected.SPSS statistical software was used to analyze and compare the differences between groups by independent sample t-test.Results After 25Gy X-ray andγ-ray irradiation,the inhibition rates of lymphocytes were(98.034±1.778)%and(97.882±1.915)%,respectively.Compared with theγirradiation group,the difference of Plt,PDW,MPV,P-LCR,PCT,pH,CD41b and CD62p between 1-day and 3-day group after 25Gy X-ray irradiation showed not statistically significance(P>0.05).Conclusion 25Gy X-ray irradiation can effectively inactivate lymphocytes in APs,and the radiation effect was equivalent withγ-ray;at the same time,there was no significant influence on the quantity of APs.

关 键 词：X射线 Γ射线 单采血小板 淋巴细胞 输血相关移植物抗宿主病 

分 类 号：R457.1[医药卫生—治疗学]