动脉栓塞用直链烷基修饰的氧化石墨烯对免疫细胞的影响  

Effects of lineaRalkyl grafted graphene oxide foRembolization on immune cells

作　　者：曾琳源 王立翔 张桂元 唐可禺 林润[1] 陈斌[1] 戴海涛[1] 杨建勇[1] 黄勇慧[1] ZENG Lin-yuan;WANG Li-xiang;ZHANG Gui-yuan(Department of Interventional Radiology,the First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]中山大学附属第一医院放射介入科,广州510080 [2]中山大学中山医学院,广州510080

出　　处：《中国临床新医学》2022年第9期799-805,共7页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：国家自然科学基金面上项目(编号:81671792);广州市科技计划项目(编号:202002030084)。

摘　　要：目的研究可作为动脉栓塞材料的直链烷基修饰氧化石墨烯(GO-C18)纳米载体对免疫细胞的影响。方法从BALB/c小鼠骨髓提取巨噬细胞培养,从BALB/c小鼠脾脏提取淋巴细胞培养并分选。对处于对数期生长期的巨噬细胞、T细胞、B细胞予以10μg/ml氧化石墨烯(GO)、10μg/ml GO-C18和等量的磷酸缓冲盐溶液(PBS)进行处理,然后通过流式细胞技术检测巨噬细胞、T细胞、B细胞的活化情况,并分析巨噬细胞的功能变化情况。将对数期的巨噬细胞予以200μg/ml、100μg/ml、50μg/ml、10μg/ml不同浓度GO、GO-C18和等量的PBS处理后检测巨噬细胞吞噬能力。建立小鼠巨噬细胞-白血病细胞(L1210细胞)共培养体系,予10μg/ml的GO、GO-C18及等量PBS处理后检测巨噬细胞吞噬能力。结果10μg/ml GO-C18处理48 h后巨噬细胞表面的CD69、CD80、CD86、信号调节蛋白α(SIRPα)、主要组组织相容性复合物Ⅱ(MHCⅡ)等标志物表达稳定,表明巨噬细胞活化、M1表型、M2表型、抗原提呈能力均未受到明显的影响。10μg/ml GO-C18处理后72 h后T细胞、B细胞表达CD69正常,T细胞、B细胞的活化均未受到明显的影响。10μg/ml GO-C18处理24 h后巨噬细胞吞噬功能未受到明显的影响。结论本研究初步证明GO-C18对巨噬细胞功能与活化以及T细胞、B细胞的活化无显著不良影响。Objective To investigate the effects of lineaRalkyl grafted graphene oxide(GO-C18)nanocarriers foRembolization on immune cells.Methods Macrophages were extracted from the bone marrow of BALB/c mice foRculture,and lymphocytes were extracted from the spleen of BALB/c mice foRculture and cell sorting.Macrophages,T cells,and B cells in logarithmic phase were treated with 10μg/ml graphene oxide(GO),10μg/ml GO-C18 and equal volume of phosphate buffeRsaline(PBS)foRa certain time.Then,the activation of macrophages,T cells and B cells were detected by flow cytometry and the function of macrophages was analysed.The macrophages in logarithmic phase were treated with different concentrations of GO,GO-C18(200μg/ml,100μg/ml,50μg/ml,10μg/ml)and equal volume of PBS foRa certain time to detect the phagocytic ability of macrophages.A mouse macrophage-leukemia cell(L1210 cell)co-culture system was established and treated with 10μg/ml GO,GO-C18 and equal volume of PBS foRa certain time to detect the phagocytic ability of macrophages.Results The expressions of markers such as CD69,CD80,CD86,signal regulatory protein alpha(SIRPα),and majoRgroup histocompatibility complexⅡ(MHCⅡ)on macrophages were stable afteRtreatment with 10μg/ml GO-C18 foR48 h,indicating that macrophage activation,M1 phenotype,M2 phenotype,and antigen-presenting ability were not significantly affected.The expressions of CD69 on T cells and B cells were stable afteRtreatment with 10μg/ml GO-C18 foR72 hours,indicating that the activations of T cells and B cells were not significantly affected.The phagocytic function of macrophages was not significantly affected afteRtreatment with 10μg/ml GO-C18 foR24 hours.Conclusion This study preliminarily demonstrates that GO-C18 has no significant adverse effects on the function and activation of macrophages and the activations of T cells and B cells.

关 键 词：直链烷基修饰氧化石墨烯 免疫细胞 动脉栓塞 

分 类 号：R318.08[医药卫生—生物医学工程]