玉米素合成通路相关基因的克隆及表达  

Cloning and Expression of Genes Related to Zeatin Synthesis Pathway

作　　者：张咏祀[1] 龙芸 郑祖平 张红梅[3] 刘小红[1] 曹丹 李仕伟 ZHANG Yongsi;LONG Yun;ZHENG Zuping;ZHANG Hongmei;LIU Xiaohong;CAO Dan;LI Shiwei(College of Life Sciences,China West Normal University,Nanchong Sichuan 637000,China;Sweetpatato and Leguminosae Germplasm Innovation and Utilization Key Laboratory of Sichuan Province,Nanchong Sichuan 637000,China;Maize Research Institute,Shanxi Academy of Agricultural Sciences,Xinzhou Shanxi 034000,China)

机构地区：[1]西华师范大学生命科学学院,四川南充637000 [2]厅市共建甘薯及特色豆科作物种质创新与利用四川省重点实验室,四川南充637000 [3]山西省农业科学院玉米研究所,山西忻州034000

出　　处：《种子》2022年第8期55-60,共6页Seed

基　　金：南充市农科院重点实验室开放基金项目(2021 CHIHL 01)。

摘　　要：高温已成为近年来世界范围内制约玉米生产的最重要的非生物胁迫因素之一,然而对玉米耐高温胁迫的分子机理的认识还十分有限。本试验选取中地88(耐高温胁迫)和先玉335(不耐高温胁迫)两个玉米品种为试验材料,在42℃高温和28℃常温两种条件下研究其花粉中玉米素合成通路相关基因的表达情况。结果表明,共有5个玉米素合成相关基因,即Zmco5、Zmit4、Zmco4b、Zmco12和Zmczg1,基因编码蛋白的氨基酸数目分别为582、364、534、528、465个。进一步对这5个基因表达蛋白作了生物信息学分析,包括常见理化性质、功能位点、磷酸化修饰、二级结构和三级结构等,结果表明,不同蛋白其生物学表型存在差异。基因表达结果显示,对于耐高温胁迫的中地88,这5个基因表达差异达到2倍以上,以常温条件为对照,Zmco5基因表达上调,其余为表达下调。但对先玉335的Zmco5和Zmczg1基因表达上调,其余3个基因表达下调。这5个基因均处于玉米素合成通路中,与细胞内玉米素的生物合成密切相关。High temperature has become one of the most important abiotic stress factors restricting maize production worldwide in recent years, but the molecular mechanism of maize resistance to high temperature stress is still very limited. In this study, two maize varieties Zhongdi 88(resistant to high temperature stress) and Xianyu 335(susceptive to temperature stress) were selected as experimental materials, in order to study the expression of genes related to zeatin synthesis pathway in pollen at 42 ℃ and 28 ℃. The results showed that five genes related to zeatin synthesis were identified, named Zmco5, Zmit4, Zmco4 b, Zmco12 and Zmczg1, with 582, 364, 534, 528 and 465 amino acids with gene-encoded protein, respectively. Bioinformatics analysis was performed on the expression proteins of the five genes, including common physical and chemical properties, functional sites, phosphorylation modification, secondary structure and tertiary structure, etc. The results showed that the biological phenotypes of different proteins were different. The results of gene expression demonstrated that the expression differences of these 5 genes in Zhongdi 88 were more than two times under the condition of normal temperature as control, the expression of Zmco5 gene was up-regulated and the other genes were down-regulated;while for Xianyu 335, the expression of Zmco5 and Zmczg1 were up-regulated, and the other three were down-regulated. These five genes were all involved in the zeatin synthesis pathway and were closely related to the biosynthesis of zeatin in cells.

关 键 词：玉米 玉米素 高温胁迫 转录组 

分 类 号：S513[农业科学—作物学]