氧化低密度脂蛋白、血清可溶性凝集素样氧化低密度脂蛋白受体-1与痛风患者亚临床动脉粥样硬化的关系  

Association of serum ox-LDL,sLOX-1 and subclinical atherosclerosis in patients with gout

作　　者：郑雪娜 聂豪 戢操 李敏[2] ZHENG Xuena;NIE Hao;JI Cao;LI Min(Department of Hematology,People's Hospital Affiliated to Hubei University of Medicine,Shiyan,Hubei 442000,China;Department of Endocrinology and Rheumatology,Shiyan City Taihe Hospital Affiliated to Hubei University of Medicine,Shiyan,Hubei 442000,China)

机构地区：[1]湖北医药学院附属人民医院血液内科,湖北十堰442000 [2]湖北医药学院附属十堰市太和医院内分泌风湿病科,湖北十堰442000

出　　处：《国际检验医学杂志》2022年第19期2375-2379,2385,共6页International Journal of Laboratory Medicine

基　　金：国家自然科学基金面上项目(81372998)。

摘　　要：目的 分析痛风患者血清可溶性凝集素样氧化低密度脂蛋白受体-1(sLOX-1)和氧化低密度脂蛋白(ox-LDL)的变化及其与患者亚临床动脉粥样硬化(AS)的关系。方法 选择68例痛风患者和43例体检健康者(对照组)作为研究对象,根据双侧颈动脉内中膜厚度(IMT)和有无动脉斑块形成,将痛风患者分为亚临床AS组(AS组,38例)和非亚临床AS组(non-AS组,30例)。用酶联免疫吸附试验测定血清sLOX-1和ox-LDL水平,记录痛风患者病程、体质量指数(BMI)、血尿酸(UA)、血脂、空腹血糖、糖化血红蛋白(HbA1c)、胰岛素抵抗指数(HOMA-IR)和超敏C反应蛋白(hs-CRP)等临床资料。分析痛风患者血清ox-LDL和sLOX-1的相关性。采用受试者工作特征(ROC)曲线及曲线下面积(AUC)评价ox-LDL和sLOX-1水平对痛风合并亚临床AS的诊断价值。采用多因素Logisitic回归分析痛风患者合并亚临床AS的相关危险因素。结果 AS组患者血清ox-LDL[42.37(20.77,181.20)ng/mL]与sLOX-1[7.66(4.43,14.03)ng/mL]水平均高于non-AS组[oxLDL:19.03(8.46,32.44)ng/mL,sLOX-1:5.70(2.41,10.87)ng/mL],non-AS组ox-LDL和sLOX-1水平高于对照组[ox-LDL:10.71(4.22,14.74)ng/mL,sLOX-1:0.98(0.49,3.18)ng/mL],差异均有统计学意义(P<0.05);痛风患者血清ox-LDL水平与sLOX-1水平呈正相关(r=0.531,P<0.001);ox-LDL诊断痛风患者合并亚临AS的AUC为0.718,sLOX-1诊断痛风患者合并亚临床AS的AUC为0.769。多因素Logistic回归分析显示,年龄、病程、三酰甘油、低密度脂蛋白胆固醇、HOMA-IR、ox-LDL是痛风患者合并亚临床AS的影响因素。结论 痛风患者血清ox-LDL、sLOX-1表达增加与患者亚临床AS的发生密切相关,二者的临床检测对痛风合并AS的早期诊断有意义。Objective To analyze the changes of serum oxidized low density lipoprotein(ox-LDL) and lipoprotein lectin-like oxidation of LDL receptors-1(sLOX-1) in patients with gout and their relationship with subclinical atherosclerosis(AS).Methods A total of 68 patients with gout and 43 healthy subjects(control group) were selected as subjects.According to the bilateral carotid intima-media thickness(IMT) and the presence or absence of plaque formation, gout patients were divided into subclinical AS group(AS group, 38 cases) and non-subclinical AS group(non-AS group, 30 cases).Serum sLOX-1 and ox-LDL levels were measured by enzyme-linked immunosorbent assay.The course of disease, body mass index(BMI),serum uric acid(UA),blood lipids, fasting blood glucose, glycosylated hemoglobin a1 c(HbA1 c),homeostasis model assessment of insulin resistance(HOMA-IR) and high-sensitivity C-reactive protein(hs-CRP) were recorded.The correlation between serum ox-LDL and SLOX-1 in patients with gout was analyzed.Receiver operating characteristic(ROC) curve and area under the curve(AUC) were used to evaluate the diagnostic value of ox-LDL and sLOX-1 levels in gout complicated with subclinical AS.Logisitic regression was used to analyze the risk factors of subclinical AS in patients with gout.Results Levels of serum ox-LDL[42.37(20.77,181.20)ng/mL] and sLOX-1 [7.66(4.43,14.03)ng/mL] in AS group were higher than those in non-AS group [oxLDL:19.03(8.46,32.44)ng/mL,sLOX-1:5.70(2.41,10.87)ng/mL],the levels of serum ox-LDL and sLOX-1 in non-AS group were higher than those in control group [ox-LDL:10.71(4.22,14.74)ng/mL,sLOX-1:0.98(0.49,3.18)ng/mL],and the differences were statistically significant(P<0.05).Serum ox-LDL level was positively correlated with sLOX-1 level in patients with gout(r=0.531,P<0.001).The AUC of ox-LDL in the diagnosis of gout patients with subclinical AS was 0.718,the AUC of sLOX-1 in the diagnosis of gout patients with subclinical AS was 0.769.Logistic regression analysis showed that age, disease duration, triglyceride, low-d

关 键 词：痛风 氧化低密度脂蛋白 可溶性凝集素样氧化低密度脂蛋白受体-1 动脉粥样硬化 

分 类 号：R589.7[医药卫生—内分泌]