结直肠癌组织LncRNA MEG3、miR-31表达及与病理特征的相关性  

Expressions of LncRNA MEG3 and miR-31 in Colorectal Cancer and Their Correlations with Pathological Characteristics

作　　者：赵芝[1] 张刚[1] 李哲丽 刘玉凤 郑少华 刘楠 ZHAO Zhi;ZHANG Gang;LI Zhe-li;LIU Yu-feng;ZHENG Shao-hua;LIU Nan(Department of Gastroenterology,the People's Hospital of Dingzhou City,Dingzhou,Hebei 073000,China;Department of Gastroenterology,the People's Hospital of Shijiazhuang City,Shijiazhuang 050000,China)

机构地区：[1]定州市人民医院消化内科,河北定州073000 [2]石家庄市人民医院消化内科,石家庄050000

出　　处：《解放军医药杂志》2022年第7期39-43,共5页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：2022年河北省医学科学研究课题计划(20220432)。

摘　　要：目的探讨结直肠癌组织长链非编码RNA母系印记基因3(LncRNA MEG3)、微小RNA-31(miR-31)表达及与病理特征的相关性。方法选取2019年1月—2021年10月我院收治的85例结直肠癌,比较癌组织与癌旁组织LncRNA MEG3与miR-31表达,应用Pearson相关性分析探讨LncRNA MEG3与miR-31的关系,采用荧光素酶报告基因检测分析LncRNA MEG3靶向调控miR-31情况,并比较不同病理特征患者LncRNA MEG3与miR-31表达,应用Spearman分析探讨LncRNA MEG3、miR-31与病理特征的相关性。结果癌组织LncRNA MEG3表达低于癌旁组织,miR-31表达高于癌旁组织(P<0.01)。LncRNA MEG3与miR-31表达呈负相关(r=-0.718,P<0.01)。荧光素酶报告基因检测结果显示,LncRNA MEG3 mimic可明显抑制WT-miR-31的荧光素酶活性(P<0.05);LncRNA MEG3组SW480细胞中miR-31表达显著降低,anti-LncRNA MEG3组SW480细胞中miR-31表达显著升高(P<0.05)。不同T分期、N分期、M分期、分化程度患者LncRNA MEG3与miR-31表达比较差异有统计学意义(P<0.01)。LncRNA MEG3表达与T分期、N分期呈负相关(r=-0.737、-0.725,P<0.01),与分化程度呈正相关(r=0.824,P<0.01);miR-31表达与T分期、N分期呈正相关(r=0.830、0.748,P<0.01),与分化程度呈负相关(r=-0.742,P<0.01)。结论结直肠癌组织中LncRNA MEG3呈低表达,miR-31呈高表达,且LncRNA MEG3可特异性结合miR-31,负向调控miR-31表达,影响结直肠癌患者病理特征。Objective To investigate expressions of long-chain noncoding RNA maternal imprinted gene 3(LncRNA MEG3)and microrna-31(miR-31)in colorectal cancer(CRC)tissues and their correlations with pathological characteristics.Methods A total of 85 patients with CRC admitted between January 2019 and October 2021 were selected,and expressions of LncRNA MEG3 and miR-31 in cancer tissues and adjacent tissues were compared.Pearson correlation analysis was used to explore correlation between LncRNA MEG3 and miR-31.Luciferase reporter gene detection was used to analyze the targeted regulation of miR-31 by LncRNA MEG3,and expressions of LncRNA MEG3 and miR-31 in patients with different pathological characteristics were compared.Spearman analysis was used to explore correlations between LncRNA MEG3 and miR-31 with pathological characteristics.Results In cancer tissues,LncRNA MEG3 expression was lower than that in adjacent tissues,while miR-31 expression was higher than that in adjacent tissues(P<0.01).LncRNA MEG3 was negatively correlated with miR-31 expression(r=-0.718,P<0.01).Result of luciferase reporter gene assay showed that LncRNA MEG3 mic could significantly inhibit the luciferase activity of WT-mir-31(P<0.05).The expression of miR-31 in SW480 cells in LncRNA MEG3 group was significantly decreased,while the expression of miR-31 in SW480 cells in anti-LncRNA MEG3 group was significantly increased(P<0.05).There were significant differences in expressions of LncRNA MEG3 and miR-31 in patients with different T stages,N stages,M stages and differentiation degrees(P<0.01).LncRNA MEG3 expression was negatively correlated with T stage and N stage(r=-0.737,-0.725,P<0.01),and positively correlated with differentiation degree(r=0.824,P<0.01).The expression of miR-31 was positively correlated with T stage and N stage(r=0.830,0.748,P<0.01),and negatively correlated with differentiation degree(r=-0.742,P<0.01).Conclusion Lowly expressed LncRNA MEG3 and highly expressed miR-31 can be found in CRC tissues.LncRNA MEG3 may specifically bind t

关 键 词：结直肠肿瘤 长链非编码RNA母系印记基因3 微小RNA-31 肿瘤分期 

分 类 号：R735.34[医药卫生—肿瘤]