肺炎支原体肺炎患儿血清SP-D、Gal-3、CCL5水平与炎症因子和预后不良的关系研究  

Study of the Relationship between Serum SP-D, Gal-3 and CCL5 Levels and Inflammatory Factors and Poor Prognosis in Children with Mycoplasma Pneumoniae Pneumonia

作　　者：董学广 刘莉 燕民 傅晓夏 李江[3] DONG Xue-guang;LIU Li;YAN Min;FU Xiao-xia;LI Jiang(Department of Clinical Laboratory,Jinan People's Hospital Afiliatedto Shandong First Medical University Jinan,Shandong 271100,China;Department of Pediatrics,Jinan People's Hospital Affiliated to Shandong First Medical University,Jinan,Shandong,271100,China;Department of Clinical Laboratory,China Japan Friendship Hospital,Beijing,10ooo0,China)

机构地区：[1]山东第一医科大学附属济南人民医院检验科,山东济南271100 [2]山东第一医科大学附属济南人民医院儿科,山东济南271100 [3]中日友好医院检验科,北京100000

出　　处：《现代生物医学进展》2022年第16期3126-3131,共6页Progress in Modern Biomedicine

基　　金：山东省自然科学基金项目(ZR2017HM081)。

摘　　要：目的:探讨肺炎支原体肺炎(MPP)患儿血清表面活性蛋白D(SP-D)、半乳糖凝集素-3(Gal-3)、C-C基序趋化因子配体5(CCL5)水平与炎症因子和预后不良的关系。方法:选取2020年1月~2021年1月我院收治的165例MPP患儿为MPP组,另选取54例健康儿童为对照组。采用ELISA法检测血清SP-D、Gal-3、CCL5、白细胞介素-6(IL-6)、IL-8水平,放射免疫法检测血清肿瘤坏死因子-α(TNF-α)水平。采用Pearson/Spearman相关系数分析MPP患儿血清SP-D、Gal-3、CCL5水平与IL-6、IL-8、TNF-α水平的相关性。通过多因素Logistic回归分析MPP患儿预后不良的影响因素。采用接受者操作特征(ROC)曲线分析血清SP-D、Gal-3、CCL5水平对MPP患儿预后不良的预测价值。结果:与对照组比较,MPP组患儿的血清SP-D、Gal-3、CCL5以及IL-6、IL-8、TNF-α水平升高(P<0.05)。MPP患儿的血清SP-D、Gal-3、CCL5水平与IL-6、IL-8、TNF-α水平均呈正相关(P<0.05)。血清SP-D、Gal-3、CCL5、IL-6、TNF-α水平较高、病变类型为大片状阴影、热程较长是MPP患儿预后不良的危险因素(P<0.05)。血清SP-D、Gal-3、CCL5三项联合预测MPP患儿预后不良的曲线下面积(AUC)为0.926,明显大于三项指标单独预测的0.842、0.794、0.771。结论:MPP患儿血清SP-D、Gal-3、CCL5水平升高,与炎症因子和预后不良密切相关,可作为MPP患儿预后不良的预测指标。Objective: To investigate the relationship between serum surfactant protein-D(SP-D), galectin-3(Gal-3) and C-C motif chemokine ligand 5(CCL5) levels and inflammatory factors and poor prognosis in children with mycoplasma pneumoniae pneumonia(MPP). Methods: A total of 165 children with MPP who were admitted to our hospital from January 2020 to January 2021 were selected as the MPP group, and 54 healthy children were selected as the control group. Serum SP-D, Gal-3, CCL5, interleukin-6(IL-6) and IL-8 levels were measured by ELISA, and serum tumor necrosis factor-α(TNF-α) level was measured by radioimmunoassay. Pearson/Spearman coefficients were used to analyze the correlation between serum SP-D, Gal-3 and CCL5 levels and IL-6, IL-8, and TNF-α levels in children with MPP. The influencing factors of poor prognosis in children with MPP were analyzed by multivariate Logistic regression,and receiver operating characteristic(ROC) curve was used to analyze the predictive value of serum SP-D, Gal-3 and CCL5 levels on poor prognosis in children with MPP. Results: Compared with the control group, serum SP-D, Gal-3, CCL5, IL-6, IL-8 and TNF-α levels in children with MPP group increased(P<0.05). The serum SP-D, Gal-3 and CCL5 levels in children with MPP were positively correlated with IL-6, IL-8 and TNF-α levels(P<0.05). Serum SP-D, Gal-3, CCL5, IL-6 and TNF-α high levels, the lesion type was large flaky shadow and long heat range were the risk factors for poor prognosis in children with MPP(P<0.05). The area under the curve(AUC) of serum SP-D, Gal-3 and CCL5 in predicting the poor prognosis of children with MPP was 0.926, which was significantly greater than 0.842, 0.794 and 0.771 predicted by the three indexes alone. Conclusion: The elevated levels of serum SP-D, Gal-3 and CCL5 in children with MPP are closely related to inflammatory factors and poor prognosis, which can be used as a predictor of poor prognosis in children with MPP.

关 键 词：儿童 肺炎支原体肺炎 炎症 预后 SP-D GAL-3 CCL5 

分 类 号：R563.15[医药卫生—呼吸系统]