盐度胁迫对弓背青鳉水通道蛋白基因转录的影响  

Effect of salinity stress on aquaporin gene transcription in Oryzias curvinotus

作　　者：黄泽胤 陈月碧 黎学友 张宁 郭昱嵩[1] 王中铎[1,2,3] 董忠典 HUANG Ze-yin;CHEN Yue-bi;LI Xue-you;ZHANG Ning;GUO Yu-song;WANG Zhong-duo;DONG Zhong-dian(Guangdong South China Sea Key Laboratory of Aquaculture for Aquatic Economic Animals,Fisheries College,Guangdong Ocean University,Zhanjiang 524088,China;Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture,Zhanjiang 524088,China;State Laboratory of Developmental Biology of Freshwater Fish,College of Life Sciences,Hunan Normal University School,Changsha 410081,China)

机构地区：[1]广东海洋大学水产学院,南海水产经济动物增养殖重点实验室,广东湛江524088 [2]广东省水产动物病害防控与健康养殖重点实验室,广东湛江524088 [3]省部共建淡水鱼类发育生物学国家重点实验室,湖南长沙410081

出　　处：《生物技术》2022年第4期415-420,共6页Biotechnology

基　　金：国家自然科学基金项目(41806195,31201996);广东海洋大学创新强校项目(2018KTSCX087,2018KQNCX110);广东海洋大学南海青年学者项目(QNXZ201807,201903)。

摘　　要：[目的]探究短期盐度胁迫对弓背青鳉(Oryzias curvinotus)水通道蛋白基因(Aquaporin family genes,aqps)转录水平的影响。[方法]以不同盐度(0、13和26)对实验室淡水养殖的弓背青鳉成鱼进行7 d的盐度胁迫,观察弓背青鳉的生存和生长,通过实时荧光定量PCR(quantitative Real-time PCR,qPCR)方法检测aqps在肝脏和鳃组织中转录水平的变化。[结果]淡水对照组和13盐度胁迫处理组弓背青鳉在实验过程中均无死亡;26盐度胁迫处理组在实验初期有2个体死亡。短期盐度胁迫未改变aqps在弓背青鳉鳃组织及aqp0、aqp1、aqp3、aqp10、aqp11在肝脏组织中的转录水平;但显著影响了aqp7和aqp12在肝脏中的转录水平:aqp12转录水平随着盐度的升高而升高,在26盐度胁迫下转录水平显著高于对照组和13盐度组(P<0.05);aqp7的转录水平则随着胁迫盐度的升高而下降,在26盐度胁迫下aqp7表达水平较对照组显著下调(P<0.05)。[结论]弓背青鳉对短期盐度胁迫具有较好的适应能力,肝脏中aqp12和aqp7可能通过调节自身转录水平参与了机体对盐度变化的适应过程。该研究有助于明晰弓背青鳉的广盐性分子机制,为将其开发成广盐性模式生物提供了基础数据。[Objective]To explore the effect of short-term salinity stress on aquaporin gene transcription level in Hainan medaka(Oryzias curvinotus).[Method]Different salinities(0,13 and 26)were used to observe the survival and growth of medaka cultured in freshwater for 7 days.The changes of aquaporin family member genes(aqps)in liver and gill were detected by quantitative Real-time PCR(qPCR).[Result]There was no death of medaka in 0 and 13 salinity groups,and two individuals died in 26 salinity stress groups at the beginning of the experiment.Short term salinity stress did not change the transcription levels of aqps in gills,and aqp0,aqp1,aqp3,aqp10 and aqp11 in liver,but significantly affected the transcription levels of aqp7 and aqp12 in liver:The transcription level of aqp12 increased with the increase of salinity,and the transcription level of 26 salinity stress was significantly higher than that of control group and 13 salinity stress group(P<0.05);The transcription level of aqp7 decreased with the increase of salinity,and the expression level of aqp7 was significantly lower than that of the control group under 26 salinity stress(P<0.05).[Conclusion]Medaka has good adaptability to short-term salinity stress.Aqp12 and aqp7 in liver may be involved in the adaptation process of organism to salinity change.This study will be helpful to clarify the broad salt molecular mechanism of medaka and provides basic data for developing it into a broad salt model organism.

关 键 词：弓背青鳉 盐度胁迫 水通道蛋白 转录水平 鳃 肝脏 

分 类 号：S917.4[农业科学—水产科学]