催产素受体在山羊颈中神经节的定位研究  

Immunohistochemical Localization of OTR in Middle Cervical Ganglion of Goats

作　　者：陈文东[1] 朱秀娟[1] 何玉鹏 王昱[1] 徐永平[2] CHEN Wen-dong;ZHU Xiu-juan;HE Yu-peng;WANG Yu;XU Yong-ping(School of Agriculture and Forestry Technology,Longnan Teachers College,Chengxian,Gansu 742500,China;College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi 712100,China)

机构地区：[1]陇南师范高等专科学校农林技术学院,甘肃成县742500 [2]西北农林科技大学动物医学学院,陕西杨凌712100

出　　处：《动物医学进展》2022年第10期39-43,共5页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31072184);陕西省农业推广项目(k332021309);陇南市科技计划项目(2021-11);甘肃省高校创新能力提升工程项目(2020B-363)。

摘　　要：为了研究山羊颈中神经节(middle cervical ganglion,MCG)的神经细胞内是否具备接受催产素(oxytocin,OT)调节的条件。取成年羊山颈中神经节,采用免疫组化SP法检测催产素受体(oxytocin receptor,OTR)在颈中神经节内各种结构上的分布特点,用Image-pro plus 6.0分析颈中神经节神经细胞和非神经细胞结构的催产素受体表达差异性。结果表明,颈中神经节内OTR免疫阳性产物分布广泛,神经细胞、过路神经纤维、血管内皮细胞、支持细胞和雪旺细胞均有不同程度的着色。OTR免疫阳性产物在神经细胞的胞质和细胞核核膜着色最深,为棕褐色,呈强阳性。大部分神经细胞胞质着色为黄褐色,呈中等阳性,这部分神经细胞胞质约占神经细胞总数的91%。血管内皮细胞、细胞膜,以及一小部分神经细胞胞质和少量神经纤维着色较浅,为淡黄色,呈弱阳性。大部分的过路神经纤维着色极浅或不着色,为阴性。图像分析表明神经细胞胞体的相对表达量与其他非神经细胞结构相比差异性极显著。OTR免疫阳性产物在山羊颈中神经节中广泛表达分布,且主要分布在颈中神经节交感节后神经细胞内。This experiment was conducted to detect the existence of oxytocin receptor(OTR)in the middle cervical ganglion(MCG)of goats.MCG was taken from mature goats,immunohistochemical SP method was used to study the distribution characteristics of OTR on MCG in goats,Image-Pro Plus 6.0 was used as the method of image analysis to evaluate the expression difference of MCG between neurons and non-neuron cells.The results showed that,OTR immunopositivity was widely distributed in MCG of goat,and different levels of staining existed in neurons,nerve fibers,vascular endothelial cells,Sertoli cells and Schwan cells.OTR immunopositive products were mainly distributed in cytoplasma and nuclear membrane of neurons,staining was dark and brown,strongly positive.Cytoplasmic staining of most neurons is yellow-brown,moderately positive,about 91%of the total number of neurons.Vascular endothelial cells,cytomembrane,and a fraction of the cytoplasma of neurons and a few nerve fibers were dyed shallow,pale yellow,weak positive.Most of the passing nerve fibers were vacuolar,stained weak or no staining,negative.Image analysis showed that the relative expression of immunoreactive product OTR was significantly higher than that of other non-neural structures(P<0.01).OTR immunopositivity was widely distributed in MCG of goat,The OTR in the MCG of goats mainly expressed in postsympathetic ganglia neurons.

关 键 词：催产素受体 颈中神经节 免疫组织化学SP法 山羊 

分 类 号：S854.59[农业科学—临床兽医学] S858.27[农业科学—兽医学]