垫状卷柏中化学成分的分离及其抑制肿瘤细胞增殖活性  

Isolation of chemical constituents from Selaginella pulvinata and their anti-proliferative activity on tumor cells

作　　者：蔡蕊[1] 于远洋 谢宁 徐强[1] 万慧慧[1] 王世盛[3,4] CAI Rui;YU Yuan-yang;XIE Ning;XU Qiang;WAN Hui-hui;WANG Shi-sheng(Analytical Instrumentation Center,Dalian University of Technology,Dalian 116024,China;Dalian Inspection and Certification Technical Service Center,Dalian 116081,China;Department of Pharmaceutical Sciences,School of Chemical Engineering,Dalian University of Technology,Dalian 116024,China;Ningbo Institute of Dalian University of Technology,Ningbo 315016,China)

机构地区：[1]大连理工大学分析测试中心,大连116024 [2]大连市检验检测认证技术服务中心,大连116081 [3]大连理工大学化工学院,大连116024 [4]大连理工大学宁波研究院,宁波315016

出　　处：《天然产物研究与开发》2022年第9期1507-1514,共8页Natural Product Research and Development

基　　金：中央高校基本科研业务费(DUT21YG124)。

摘　　要：利用质谱引导的自动纯化分离系统对垫状卷柏95%乙醇提取物的乙酸乙酯萃取部分进行选择性分离纯化双黄酮类、炔酚类化合物。并利用MS、^(1)H NMR、^(13)C NMR、2D NMR等光波谱分析方法确定结构,结果得到四个双黄酮类化合物,分别为扁柏双黄酮(1)、异柳杉双黄酮(2)、苏铁双黄酮(3)、穗花杉双黄酮(4),七个炔酚类化合物,分别为selaginellin(5)、selaginpulvilin A(6)、selaginpulvilin B(7)、selaginellin O(8)、selaginellin E(9)、selaginellin A(10)、selaginellin B(11)。采用MTT法评价了化合物1~11体外抑制人非小细胞肺癌细胞(NSCLC)H322增殖活性,结果表明,除化合物4和7外,其他化合物对人非小细胞肺癌细胞H322有不同程度的增殖抑制活性,其中化合物3和10抑制作用较强,IC_(50)值分别为16.70和9.54μmol/L。Targeting isolation of biflavonoids and selaginellin derivatives from Selaginella pulvinata(Hook.&Grev.)Maxim.was investagated by was performed on a Waters preparative LC-MS autopurification system and the ethyl acetate part of the 95%ethanol extract of S.pulvinata was separated and purified.The chemical structures of the separated compounds were identified by MS,^(1)H NMR,^(13)C NMR,2D NMR and spectral analysis methods.Four biflavonoids were isolated and identified as hinokiflavone(1),isocryptomerin(2),sotetsuflavone(3)and amentoflavone(4).Seven selaginellin derivatives were obtained and identified as selaginellin(5),selaginpulvilin A(6),selaginpulvilin B(7),selaginellin O(8),selaginellin E(9),selaginellin A(10)and selaginellin B(11).The MTT assay was used to evaluate the inhibitory effect of compounds 1~11 on the proliferation of human non-small cell lung cancer cell line(NSCLC)H322 in vitro.The results showed that compounds 1,2,3,5,6,8,9,10 and 11 had different degrees of inhibitory activity on human NSCLC cell line H322.Compounds 3 and 10 had stronger inhibitory activity on human NSCLC cell line H322,with IC_(50) values of 16.70μmol/L and 9.54μmol/L,respectively.

关 键 词：垫状卷柏 分离纯化 双黄酮 炔酚 肿瘤细胞增殖抑制 

分 类 号：R932[医药卫生—生药学]