左卡尼汀对模拟高原环境下大鼠睾丸形态学及超微结构影响的研究  

Effects of L-carnitine on the morphology and ultrastructure of rat testis in a simulated high altitude environment

作　　者：马刚 张凯[1,2,3] 张斌 张春雷[1] 常德辉[1,3] Ma Gang;Zhang Kai;Zhang Bin;Zhang Chun-lei;Chang De-hui(Department of Urology,The 940th Hospital of Joint Logistics Support Force of People's Liberation Army,Lanzhou 730050,China;Key Laboratory of Stem Cells and Gene Drugs,The 940th Hospital of Joint Logistics Support Force of People's Liberation Army,Lanzhou 730050,China;The First Clinical Medical College,Gansu University of Traditional Chinese Medicine,Lanzhou 730000,China)

机构地区：[1]中国人民解放军联勤保障部队第九四○医院泌尿外科,甘肃兰州730050 [2]中国人民解放军联勤保障部队第九四○医院甘肃省干细胞与基因药物重点实验室,甘肃兰州730050 [3]甘肃中医药大学第一临床医学院,甘肃兰州730000

出　　处：《兰州大学学报（医学版）》2022年第6期26-30,34,共6页Journal of Lanzhou University（Medical Sciences）

基　　金：全军保健专项科研基金资助项目(21BJZ43);全军计生专项科研基金资助项目(21JSZ13);中国人民解放军联勤保障部队第九四○医院专项培育基金资助项目(2021yxky017)。

摘　　要：目的 研究抗氧化剂左卡尼汀(LC)对模拟高原环境下大鼠睾丸组织损伤的保护作用及其机制。方法 将Wistar雄性大鼠随机分为平原组、高原组、高原+生理盐水(NS)组和高原+LC组,每组10只。平原组置于当地海拔1.5 km舱外饲养,其余各组均置于模拟海拔6.0 km的低压氧舱内饲养14 d。高原+LC组和高原+NS组分别腹腔注射100 mg/(kg·d) LC注射液和同体积NS。苏木精-伊红染色法观察睾丸组织形态变化;透射电镜观察睾丸组织细胞核、线粒体、内质网等超微结构变化。结果 足剂量LC能明显减轻模拟高原环境下睾丸组织损伤的形态学改变;能够保持模拟高原环境下睾丸组织细胞核、线粒体、内质网等超微结构完整性。结论 足剂量LC干预可有效减轻及改善模拟高原低压低氧环境下成年繁殖期大鼠睾丸组织损伤的形态学及超微结构改变。Objective To study the protective effect and mechanism of the antioxidant L-carnitine(LC) on testicular tissue injury in rats under a simulated high altitude environment. Methods Male Wistar rats were randomly divided into plain group, plateau group, plateau+normal saline(NS) group and plateau+LC group, with10 rats in each. The plain group was raised outside the cabin at an altitude of 1.5 km, and the other groups in a hypobaric oxygen chamber at a simulated altitude of 6.0 km for 14 d. The plateau+LC group and the plateau+NS group were intraperitoneally injected with 100 mg/(kg·d) LC injection and a same volume of NS, respectively. The morphological changes of testis tissue were observed by HE staining, ultrastructural changes such as nucleus, mitochondria and endoplasmic reticulum of testis tissue were observed by transmission electron microscope. Results The results showed that a sufficient dose of LC could significantly reduce the morphological changes of testicular tissue damage in a simulated plateau environment, could maintain the ultrastructure of testicular tissue such as nucleus, mitochondria, and endoplasmic reticulum in a simulated plateau environment in its completeness. Conclusion A sufficient dose of antioxidant LC intervention can effectively reduce and improve the morphological and ultrastructural changes of testicular tissue injury in adult reproductive rats in a simulated plateau hypobaric and hypoxic environment.

关 键 词：左卡尼汀 高原低氧 睾丸 超微结构 

分 类 号：R698.2[医药卫生—泌尿科学]