纤维母细胞生长因子3对前丘脑γ-氨基丁酸能抑制性轴突的排斥作用  

Repulsive effects of FGF3 on the inhibitory GABAergic axons in the prethalamus

作　　者：黄红 余舒扬 张蔚云 肖俐 徐贞刚 刘芳 Huang Hong;Yu Shuyang;Zhang Weiyun;Xiao Li;Xu Zhengang;Liu Fang(Dept of Cell Biology,School of Basic Medical Sciences,Nanchang University,Nanchang 330031)

机构地区：[1]南昌大学医学部基础医学院细胞生物学教研室,南昌330031

出　　处：《安徽医科大学学报》2022年第10期1574-1578,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:32160188)。

摘　　要：目的研究纤维母细胞生长因子3(FGF3)对前丘脑γ-氨基丁酸能(GABA)抑制性神经元轴突的导向作用,探讨FGF3对丘脑神经网络形成的影响。方法采用免疫荧光法检测FGF3及前丘脑GABA标志基因在鸡胚间脑的表达及定位。采用DiI示踪法检测早期前丘脑GABA抑制性神经元轴突走向。三维凝胶共培养实验证明FGF3是否对早期前丘脑GABA抑制性神经纤维具有导向作用。结果前丘脑GABA细胞与FGF3+的下丘脑相邻,位于下丘脑上方。DiI示踪实验显示,在鸡胚发育的第6天(E6),前丘脑GABA抑制性神经纤维已伸展进入背侧丘脑。与空白对照组比较,FGF3不仅能显著促进前丘脑GABA抑制性神经纤维的生长(P<0.01),并且排斥新生前丘脑轴突向背侧丘脑方向延伸,前丘脑轴突的数量在FGF3一侧的数量明显少于对侧(P<0.01)。并且,FGF3对前丘脑轴突的导向功能可被FGF信号通路抑制剂SU5402所阻滞。结论表达在下丘脑的轴突化学导向分子FGF3对相邻前丘脑GABA抑制性神经纤维的路径选择具有导向作用。Objective To study the guidance effects of fibroblast growth factoR3(FGF3)on the prethalamicγ-aminobutyric acid(GABA)inhibitory axons,and to explore the effect of FGF3 on the formation of thalamic axonal network.Methods Expression and localization of FGF3 and prethalamic GABA markeRgene in chicken embryonic diencephalon were detected by immunofluorescence.Early orientation of GABA axons was tracked by DiI.Three-dimensional gel co-culture experiment was carried out to investigate guidance effects of FGF3 on early prethalamic GABA inhibitory axons.Results Prethalamic GABA cells were adjacent to the FGF3+hypothalamas,located above the hypothalamus.DiI tracing experiments revealed that prethalamic GABA inhibitory axons had already extended into the thalamus at E6.Compared with the blank control group,FGF3 not only significantly promoted the growth of prethalamic GABA inhibitory nerve fibers in the prethalamus,but also repelled the newborn prethalamic axons to the dorsal thalamus.The numbeRof prethalamic axons was significantly less in proximal section(towards FGF3 beads)than that in distal section(away from FGF3 beads)(P<0.01).Moreover,the guidance effects of FGF3 on prethalamic axons could be blocked by the FGF pathway inhibitoRSU5402.Conclusion FGF3,an axon guidance molecule expressed in hypothalamus,exerts guidance effects on the pathway selection of adjacent prethalamic GABA inhibitory nerve fibers.

关 键 词：纤维母细胞生长因子3 GABA神经元 轴突导向 

分 类 号：Q189[生物学—神经生物学]