高效液相色谱法测定复方香芍口服液中芍药苷含量  

Content Determination of Paeoniflorin in Compound Xiangshao Oral Liquid by HPLC

作　　者：丁家昱 韩宪忠 番正 夏荣松 袁文霄 DING Jiayu;HAN Xianzhong;PAN Zheng;XIA Rongsong;YUAN Wenxiao(Chongqing General Hospital·Chongqing Hospital of University of Chinese Academy of Sciences,Chongqing,China 400014;College of Traditional Chinese Medicine,Chongqing Medical University,Chongqing,China 400016)

机构地区：[1]重庆市人民医院·中国科学院大学重庆医院,重庆400014 [2]重庆医科大学中医药学院,重庆400016

出　　处：《中国药业》2022年第19期74-76,共3页China Pharmaceuticals

基　　金：重庆市卫生和计划生育委员会2017年度中医药科技项目[ZY201702044]。

摘　　要：目的建立测定复方香芍口服液中芍药苷含量的高效液相色谱法。方法色谱柱为Waters SunFire C_(18)柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.05%磷酸二氢钾溶液(15∶85,V/V),流速为1.0 mL/min,柱温为30℃,检测波长为230 nm,进样量为10μL。结果芍药苷进样量在0.059~1.180μg范围内与峰面积积分值线性关系良好(r=0.9997,n=5);精密度、稳定性、重复性试验的RSD均小于2%;平均加样回收率为98.64%,RSD为0.72%(n=9)。结论该方法简便快速、专属性强、重复性好,适用于复方香芍口服液中芍药苷的含量测定。Objective To establish a high-performance liquid chromatography(HPLC)method for the content determination of paeoniflorin in Compound Xiangshao Oral Liquid.Methods The chromatographic column was Waters SunFire C_(18)column(250 mm×4.6 mm,5μm),the mobile phase was acetonitrile-0.05%potassium dihydrogen phosphate solution(15∶85,V/V),the flow rate was 1.0 mL/min,the column temperature was 30℃,the detection wavelength was 230 nm,and the injection volume was 10μL.Results The linear range of paeoniflorin was 0.059-1.180μg(r=0.9997,n=5).The RSDs of precision,stability and repeatability tests were lower than 2%.The average recovery of paeoniflorin was 98.64%with an RSD of 0.72%(n=9).Conclusion The method is simple,rapid,specific and repeatable,which is suitable for the content determination of paeoniflorin in Compound Xiangshao Oral Liquid.

关 键 词：复方香芍口服液 芍药苷 高效液相色谱法 含量测定 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学] R286.0