牛麝开窍散质量标准初探  

Quality Standard of Niushekaiqiao Powder

作　　者：崔妮 卫向锋 张红[2] 孙婷婷[2] 陈霄 杜晓刚 CUI Ni;WEI Xiangfeng;ZHANG Hong;SUN Tingting;CHEN Xiao;DU Xiaogang(Xi′an TCM Hospital of Encephalopathy,Xi′an,Shaanxi,China 710032;Chinese Medicine Research Institute of Shaanxi Institute of Traditional Chinese Medicine,Xi′an,Shaanxi,China 710031)

机构地区：[1]西安中医脑病医院,陕西西安710032 [2]陕西省中医药研究院中药研究所,陕西西安710031

出　　处：《中国药业》2022年第19期81-84,共4页China Pharmaceuticals

基　　金：中国民族医药学会科研项目[2020ZY116-410601];中医药传承创新暨“秦药”开发重点科学研究项目[2021-02-22-019]。

摘　　要：目的建立牛麝开窍散的质量标准。方法采用薄层色谱(TLC)法对处方中当归、黄芪、人工牛黄、石菖蒲的特征成分进行定性鉴别,采用高效液相色谱-蒸发光散射检测器(HPLC-ELSD)法测定人工牛黄中主要有效成分胆酸含量。结果建立的TLC法重复性好,特征斑点清晰,且阴性对照无干扰;胆酸进样量在0.87~2.03μg范围内,进样量的对数值与峰面积的对数值线性关系良好(r=0.9998,n=5);平均加样回收率为101.78%,RSD为3.33%(n=6)。结论所建立的方法简单可行、结果准确、专属性强,可用于牛麝开窍散的质量控制。Objective To establish the quality standard of Niushekaiqiao Powder.Methods The characteristic components inAngelicae Sinensis Radix,Astragali Radix,Bovis Calculus Artifactus and Acori Tatarinowii Rhizoma in the prescription werequalitatively identified by the thin-layer chromatography(TLC)method,and the content of cholic acid,the main effectivecomponent in Bovis Calculus Artifactus,was determined by the high-performance liquid chromatography-evaporative light-scattering detector(HPLC-ELSD).Results The established TLC had good repeatability,clear characteristic spots and nointerference from the negative reference.The linear range of cholic acid was 0.87-2.03μg(r=0.9998,n=5).The averagerecovery of cholic acid was 101.78%with an RSD of 3.33%(n=6).Conclusion The established method is simple,feasible,accurate and specific,which can be used for the quality control of Niushekaiqiao Powder.

关 键 词：牛麝开窍散 薄层色谱法 高效液相色谱-蒸发光散射检测器法 胆酸 质量标准 

分 类 号：R932[医药卫生—生药学] R284.1[医药卫生—药学] R286.0