lncRNA-HMGCS1的靶基因预测及信号通路富集分析  

Target Gene Prediction and Signal Pathway Enrichment Analysis of lncRNA-HMGCS1

作　　者：肖杨[1] 蒋振[1] XIAO Yang;JIANG Zhen(Department of Biochemistry and Molecular Biology,School of Basic Medicine and Forensic Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China)

机构地区：[1]川北医学院基础医学与法医学院生物化学与分子生物学教研室,四川南充637000

出　　处：《医学信息》2022年第17期7-11,共5页Journal of Medical Information

基　　金：南充市科技局项目(编号:20SXJCQN0004、18SXHZ0281)。

摘　　要：目的 运用生物信息学方法,分析长链非编码RNA-HMGCS1在细胞内的调控靶基因及信号通路,并对其进行生物学功能注释,阐述lncRNA-HMGCS1在细胞内的功能作用。方法 在miRDB数据库和Starbase3.0数据库中分别以lncRNA-HMGCS1作为目标基因,搜索与其结合的所有miRNAs,将2个数据库搜索的结果取交集,得到候选miRNAs。在Starbase3.0数据库中预测miRNAs的靶基因,并在DAVID数据库进行GO功能注释和Pathway通路富集。结果 miRDB数据库预测到95个miRNA与lncRNA-HMGCS1相作用,Starbase3.0数据库预测到167个miRNA与之相作用,选取2个数据库的交集共14个miRNA;GO功能注释显示14个miRNA的靶基因主要分布在转录调控、转录因子结合及细胞双链DNA结合等方面,生物学过程主要分布在细胞内转运,内质网-高尔基复合体物质转运等功能方面,信号通路富集结果主要分布在细胞衰老、细胞节律、细胞长时程增强以及泛素介导的蛋白降解方面。结论 长链非编码RNA-HMGCS1调控的细胞内靶基因及信号通路主要包括内质网-高尔基复合体物质转运、细胞衰老、细胞节律、细胞长时程增强以及泛素介导的蛋白降解等方面,进而调控细胞的生理和病理过程。Objective To analyze the regulatory target genes and signaling pathways of long non-coding RNA-HMGCS1 in cells by bioinformatics methods,and to annotate its biological function,and to elucidate the function of lncRNA-HMGCS1 in cells.Methods In the miRDB database and the Starbase3.0database,lncRNA-HMGCS1 was used as the target gene to search for all miRNAs bound to it,and the results of the two database search were intersected to obtain candidate miRNAs.The target genes of miRNAs were predicted in the Starbase3.0 database,and GO function annotation and Pathway pathway enrichment were performed in the DAVID database.Results A total of 95 miRNAs were predicted to interact with lncRNA-HMGCS1 in miRDB database,and 167 miRNAs were predicted to interact with lncRNA-HMGCS1 in Starbase3.0 database.A total of 14 miRNAs were selected from the intersection of the two databases.GO functional annotation showed that the target genes of 14 miRNAs were mainly distributed in transcriptional regulation,transcription factor binding and cell double-stranded DNA binding.Biological processes were mainly distributed in intracellular transport,endoplasmic reticulum-Golgi complex material transport and other functions.Signal pathway enrichment results were mainly distributed in cell senescence,cell rhythm,cell long-term enhancement and ubiquitin-mediated protein degradation.Conclusion The intracellular target genes and signaling pathways regulated by long non-coding RNA-HMGCS1 mainly include endoplasmic reticulum-Golgi complex material transport,cell senescence,cell rhythm,cell long-term potentiation and ubiquitin-mediated protein degradation,thereby regulating the physiological and pathological processes of cells.

关 键 词：生物学功能 靶基因 lncRNA-HMGCS1 内质网-高尔基复合体 

分 类 号：Q343.1[生物学—遗传学]