ELISA法和TRUST法在梅毒检测中的应用  

Application of ELISA and TRUST in Syphilis Detection

作　　者：曾冬梅 ZENG Dong-mei(Department of Laboratory,Shangyou County Center for Disease Control and Prevention,Shangyou 341200,Jiangxi,China)

机构地区：[1]上犹县疾病预防控制中心检验科,江西上犹341200

出　　处：《医学信息》2022年第17期138-140,共3页Journal of Medical Information

摘　　要：目的 研究ELISA法和TRUST法在梅毒检测中的应用价值。方法 选取2020年4月-2021年4月我中心疑似梅毒感染的128例患者作为研究对象,患者均进行甲苯胺红不加热法(TRUST)、酶联免疫吸附法(ELISA)检测,以梅毒螺旋体抗体明胶颗粒凝集试验(TPPA)检测结果作为金标准,观察TPPA检测结果、TRUS和ELISA检测方法诊断准确率、漏诊率、误诊率、诊断效能(敏感度、特异度、阳性预测值、阴性预测值),以及不同分期TRUS和ELISA检测方法阳性率。结果 128例患者中TPPA阳性112例,阴性16例;TRUST检测诊断准确率为49.11%,低于ELISA检测的92.86%,TRUST检测的漏诊率为30.36%、误诊率为20.54%,均高于ELISA检测的5.36%、1.79%,差异有统计学意义(P<0.05);ELISA检测敏感度为94.54%、阳性预测值为98.11%,均高于TRUST检测的75.13%、74.29%,差异有统计学意义(P<0.05);两种检测方法特异度、阴性预测值比较,差异无统计学意义(P>0.05);ELISA检测潜伏期梅毒阳性率为92.30%,高于TRUST检测的76.92%,差异有统计学意义(P<0.05);ELISA检测一期梅毒、二期梅毒、三期梅毒的检出阳性率分别为97.22%、98.27%、100.00%与TRUST检测的94.44%、94.82%、100.00%比较,差异无统计学意义(P>0.05)。结论 ELISA法和TRUST法在梅毒检测中均具有一定的价值,但是与TRUST法比较,ELISA法检测敏感度、准确率均较高,可提高临床梅毒筛查和确诊。Objective To study the application value of ELISA and TRUST in syphilis detection.Methods A total of 128 patients with suspected syphilis infection in our center from April 2020 to April 2021 were selected as subjects.All patients were tested by toluidine red unheated method(TRUST) and enzyme-linked immunosorbent assay(ELISA).The results of treponema pallidum antibody gelatin particle agglutination test(TPPA) were used as the gold standard to observe the diagnostic accuracy,missed diagnosis rate,misdiagnosis rate,diagnostic efficacy(sensitivity,specificity,positive predictive value,negative predictive value) of TPPA test results,TRUS and ELISA test methods,as well as the positive rates of TRUS and ELISA test methods at different stages.Results Among 128 patients,TPPA was positive in 112 cases and negative in 16 cases,the diagnostic accuracy of TRUST detection was 49.11%,which was lower than 92.86% of ELISA detection,the missed diagnosis rate of TRUST detection was 30.36%,and the misdiagnosis rate was 20.54%,which were higher than 5.36% and 1.79% of ELISA detection,the difference was statistically significant(P0.05).The positive rate of latent syphilis detected by ELISA was 92.30%,which was higher than 76.92% detected by TRUST,and the difference was statistically significant(P 0.05).Conclusion Both ELISA and TRUST have certain value in syphilis detection,but compared with TRUST,ELISA has higher sensitivity and accuracy,which can improve clinical syphilis screening and diagnosis.

关 键 词：ELISA法 TRUST法 梅毒 梅毒螺旋体 

分 类 号：R759.1[医药卫生—皮肤病学与性病学] R446.6[医药卫生—临床医学]