天麻素对2型糖尿病大鼠种植体周围骨组织影响的初步研究  

Effect of gastrodin on bone tissue around implants in type 2 diabetic rats

作　　者：张静怡 李风兰[1] 王惠[1] 李毅[1] Zhang Jingyi;Li Fenglan;Wang Hui;Li Yi(Department of Prosthodontics,Shanxi Provincial People's Hospital&The Fifth Clinical Medical College of Shanxi Medical University,Taiyuan 030000,China)

机构地区：[1]山西医科大学第五临床医学院·山西省人民医院口腔修复科,太原030000

出　　处：《中华口腔医学杂志》2022年第9期938-945,共8页Chinese Journal of Stomatology

基　　金：山西省应用基础研究计划(201901D111442);山西省重点研发计划(指南)项目(201703D321027-2)。

摘　　要：目的探讨天麻素对2型糖尿病大鼠体内氧化应激及种植体周围骨组织的影响,以期为2型糖尿病患者种植体骨结合不良问题的新药开发提供依据。方法40只5周龄SD大鼠分为对照组(10只)和造模组(30只)。造模组高脂高糖饲料喂养4周后,腹腔注射35 mg/kg剂量的链脲佐菌素行2型糖尿病造模。造模成功大鼠分为糖尿病组和天麻素组(每组各10只)。所有大鼠双侧胫骨干骺端各植入1枚纯钛种植体。术后每日天麻素组大鼠灌胃13.6 mg/kg天麻素,对照组、糖尿病组给予同等剂量生理盐水。术后每两周检测血糖。术后4、8周(每组每个时间点5只)取材,检测各组大鼠血清丙二醛和超氧化物歧化酶(superoxide dismutase,SOD)含量;免疫组化法分析抗凋亡蛋白[B细胞淋巴瘤/白血病-2(B cell lymphoma/lewkmia-2,bcl-2)蛋白]与凋亡蛋白[bcl-2关联X(bcl-2-associated X,bax)蛋白]表达情况;HE染色及显微CT扫描分析种植体周围骨结合情况,包括骨体积分数(bone volume/tissue volume,BV/TV)、连接密度(connection density,Conn.D)、骨小梁数量(trabecular number,Tb.N)和骨小梁厚度(trabecular thickness,Tb.Th)和骨小梁分离度(trabecular separation/spacing,Tb.sp)。结果术后2、4、6、8周天麻素组血糖值[(12.98±2.53)、(13.64±1.96)、(14.58±3.40)和(12.84±2.82)mmol/L]均比相同时间点糖尿病组[(20.97±2.27)、(17.94±3.76)、(23.66±2.90)和(21.22±2.67)mmol/L]显著降低(P<0.017),但两组血糖均显著高于对照组(P<0.017)。术后4和8周天麻素组丙二醛浓度[(5.21±1.60)和(3.47±1.17)nmol/ml]均显著小于相同时间点糖尿病组[(11.42±5.11)和(7.31±1.37)nmol/ml](P<0.017);天麻素组SOD活力[(42.89±6.00)和(53.12±9.73)U/ml]均显著大于相同时间点糖尿病组[(27.09±6.11)和(32.08±2.97)U/ml](P<0.017)。免疫组化染色显示,术后4和8周天麻素组种植体周围骨组织bcl-2蛋白表达均显著大于相同时间点糖尿病组(P<0.017);bax蛋白表达均显著小于相�Objective To study the effect of gastrodin on oxidative stress and bone tissue around implants in type 2 diabetes mellitus rats(T2DM),so as to provide therapeutic basis for the problems of poor osseointegration and long repair period of implants in type 2 diabetic patients,to provide the basis for the development of new therapeutic drugs.Methods Forty SD rats aged 5 weeks were divided into control group(n=10)and model group(n=30).The model group was fed with high glycolipid diet for 4 weeks and injected with 35 mg/kg streptozocin.The T2DM were divided into diabetic group(n=10)and gastrodin group(n=10).Pure screw titanium implants were placed in the metaphysis of both tibia.Rats in gastrodin group were given gastrodin 13.6 mg/kg everyday,while the control group and diabetic group were given the same dose of saline solution.Blood glucose was measured every two weeks after operation in all rats.After 4 and 8 weeks,the contents of serum malondialdehyde(MDA)and superoxide dismutase(SOD)were measured to analyze the oxidative stress level in rats.The expression of anti-apoptosis protein[B cell lymphoma/lewkmia-2(bcl-2)]and apoptosis protein[bcl-2-associated X protein(bax)]was analyzed by immunohistochemistry.HE staining and micro-CT scanning were used to analyze the osseointegration around the implants.Results The blood glucose of gastrodin[(12.98±2.53),(13.64±1.96),(14.58±3.40)and(12.84±2.82)mmol/L]were significantly lower than that of diabetic group[(20.97±2.27),(17.94±3.76),(23.66±2.90)and(21.22±2.67)mmol/L]at corresponding time point(P<0.017).Four and 8 weeks following surgery,the MDA concentration in gastrodin group[(5.21±1.60)and(3.47±1.17)nmol/ml]was significantly lower than that in diabetic group[(11.42±5.11)and(7.31±1.37)nmol/ml]at the corresponding time points(P<0.017),while SOD activity in gastrodin group[(42.89±6.00)and(53.12±9.73)U/ml]was significantly higher than those in diabetic group[(27.09±6.11)and(32.08±2.97)U/ml]at the corresponding time points(P<0.017).Immunohistochemistry showed tha

关 键 词：牙种植 糖尿病 骨结合 氧化性应激 凋亡调节蛋白质类 天麻素 

分 类 号：R285.5[医药卫生—中药学]