纳布啡调控Wnt/β-catenin信号通路抑制瑞芬太尼诱导的痛觉过敏  

Nalbuphine Regulates Wnt/β-catenin Pathway to Inhibit Remifentanil-induced Hyperalgesia

作　　者：任聪 刘海萍[2] REN Cong;LIU Haiping(Jiamusi University,Jiamusi City,Heilongjiang Province 154000;不详)

机构地区：[1]佳木斯大学,黑龙江省佳木斯市154000 [2]佳木斯大学附属第一医院麻醉科

出　　处：《医学理论与实践》2022年第19期3241-3244,共4页The Journal of Medical Theory and Practice

摘　　要：目的:研究纳布啡对瑞芬太尼诱导的痛觉过敏的作用及机制。方法:将大鼠随机分为对照组(S组)、切口痛模型组(I组)、瑞芬太尼+切口痛模型组(R+I组)和纳布啡+瑞芬太尼+切口痛模型组(N+R+I组);I组、R+I组和N+R+I组行足底切口术建立切口痛模型,R+I组和N+R+I组造模前静脉输注瑞芬太尼1μg/(kg·min),N+R+I组于瑞芬太尼输注前静脉注射纳布啡0.6mg/kg。分别于造模前24h(T0)和造模后2h、6h、24h、48h(T1~4)测定热刺激缩爪反应潜伏期(PWL)和机械刺激缩爪反应阈值(PWT)。检测脊髓组织TNF-α和IL-1β及NR1、NR2A、NR2B、Wnt3a、β-catenin蛋白的表达水平。结果:与S组比较,I组、R+I组和N+R+I组T1~T4时PWL和PWT值均明显降低,TNF-α、IL-1β及NR1、NR2B、Wnt3a、β-catenin蛋白水平明显升高(P<0.05);与I组比较,R+I组T1~T4时PWL和PWT值均明显降低,TNF-α、IL-1β及NR1、NR2B、Wnt3a、β-catenin蛋白水平明显升高(P<0.05);与R+I组比较,N+R+I组T2~T4时PWL值和PWT值均明显升高,TNF-α、IL-1β及NR1、NR2B、Wnt3a、β-catenin蛋白水平明显降低(P<0.05)。结论:纳布啡可通过抑制Wnt/β-catenin通路,抑制N-甲基-D-天冬氨酸(NMDA)受体活化和炎性因子上调,从而预防瑞芬太尼诱导的痛觉过敏。Objective:To study the effect and mechanism of nalbuphine on hyperalgesia induced by remifentanil.Methods:The rats were randomly divided into control group(S group),incision pain model group(I group),remifentanil+incision pain model group(R+I group)and nalbuphine+remifentanil+incision pain model group(N+R+I group);plantar incision was performed in I,R+I and N+R+I groups to establish incision pain model.Remifentanil was intravenously injected 1μg/(kg·min)before modeling in R+I and N+R+I groups,and nalbuphine was intravenously injected 0.6mg/kg before remifentanil infusion in N+R+I group.PWL and PWT were determined at 24 hours before modeling(T0)and 2,6,24,48 hours after modeling(T1~4),respectively.The expression levels of TNF-αand IL-1β,NR1,NR2A,NR2B,Wnt3a andβ-catenin in spinal cord were detected.Results:Compared with S group,the PWL and PWT values were significantly decreased in I,R+I and N+R+I groups at T1~T4,while TNF-α,IL-1β,NR1,NR2B,Wnt3a andβ-catenin levels were significantly increased(P<0.05).Compared with I group,the PWL and PWT values in R+I group were significantly decreased during T1~T4,while TNF-α,IL-1β,NR1,NR2B,Wnt3a andβ-catenin protein levels were significantly increased(P<0.05).Compared with R+I group,the PWL and PWT values of N+R+I group were significantly increased at T2~T4,while the levels of TNF-α,IL-1β,NR1,NR2B,Wnt3a andβ-catenin were significantly decreased(P<0.05).Conclusion:Nalbuphine can prevent hyperalgesia induced by remifentanil by inhibiting Wnt/β-catenin pathway,NMDA receptor activation and inflammatory factor upregulation.

关 键 词：纳布啡 瑞芬太尼 切口痛 痛觉过敏 WNT/Β-CATENIN 

分 类 号：R971[医药卫生—药品]