种子呼吸检测方法及其应用研究进展  

Advances in seed respiration detection and its application

作　　者：高璐 李香格 祁亨年[1] 臧影 贾良权 赵光武[2] 唐琦哲 郑雯 GAO Lu;LI Xiangge;QI Hengnian;ZANG Ying;JIA Liangquan;ZHAO Guangwu;TANG Qizhe;ZHENG Wen(School of Information Engineering,Huzhou University,Huzhou 313000,Zhejiang,China;College of Advanced Agricultural Science,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]湖州师范学院信息工程学院,浙江湖州313000 [2]浙江农林大学现代农学院,浙江杭州311300

出　　处：《浙江农林大学学报》2022年第5期1133-1143,共11页Journal of Zhejiang A&F University

基　　金：湖州市公益性应用研究项目(2021GZ30,2021GZ23);浙江省教育厅一般科研项目(Y201941626);国家自然科学基金资助项目(31701512)。

摘　　要：种子呼吸反映了种子的多种内在属性和生理生化特征,种子呼吸检测方法的进展对种子呼吸代谢研究具有重要意义。本文从种子呼吸检测方法及其应用2个方面进行了综述,重点综述了小篮子法、瓦氏微量法、Clark氧电极法、红外线二氧化碳分析仪法、氧传感技术检测法(Q技术)和可调谐二极管激光吸收光谱(TDLAS)技术检测法等常用种子呼吸检测方法的工作原理、主要检测对象及操作方法,总结了以上种子呼吸检测方法的优缺点及其适用范围。讨论了种子呼吸检测方法在种子呼吸代谢、种子储藏和种子活力测定等几个方面的研究应用,重点阐述了种子呼吸检测技术与种子活力相关的研究进展。根据研究现状对种子呼吸相关检测方法及其研究和应用方向进行了展望:(1)基于TDLAS技术等光学检测技术有望研制出灵敏度更高、操作更为简单的种子呼吸检测方法及装备;(2)种子呼吸代谢及其影响因素研究有利于丰富和深入揭示种子呼吸代谢过程理论;(3)开展种子呼吸指标和种子活力参数研究,为探寻能够将种子呼吸强度作为有效判定种子活力指标提供重要参数支持。Seed respiration reflects a variety of internal properties and physiological and biochemical characteristics of seeds. The development of seed respiration detection methods is of great significance to the study of seed respiration metabolism. The methods of seed respiration detection and their application were reviewed. The focus of the review included working principle, main detection objects and operation principle of common seed respiration detection methods, such as small-skep-method, Warburg trace method, Clark oxygen electrode method, infrared CO2 analyzer method, oxygen sensing technology(Q) and tunable diode laser absorption spectroscopy(TDLAS). The advantages, disadvantages and applicable scope of the above seed respiration detection methods were summarized. Secondly, the research and application of seed respiration detection methods in seed respiration metabolism, seed storage and seed vigor determination were discussed,with emphasis on the research progress of the correlation between seed respiration detection technology and seed vigor. Finally, based on the research status, seed respiration detection methods, their research and application direction were prospected:(1) It is expected to develop a seed respiration detection method and equipment with higher sensitivity and simpler operation based on optical detection technology such as TDLAS.(2) The study of seed respiration metabolism and its influencing factors is helpful to enrich and deeply reveal the theory of seed respiration metabolism.(3) Research on seed respiration index and seed vigor parameters should be carried out to provide important parameters to explore the possibility of using seed respiration intensity as an effective indicator to judge seed vigor.

关 键 词：种子 种子呼吸检测方法 呼吸代谢 种子储藏 种子活力 

分 类 号：S330.3[农业科学—作物遗传育种]