Phytohormones Accumulation and Distribution in Shoots and Roots of Haploid,Diploid and Tetraploid Barley Seedlings Derived from Microspore Culture  

作　　者：Longhua Zhou Ting He Jing Li Guimei Guo Yingbo Li Hongwei Xu Runhong Gao Linli Huang Yifei Wang Ruiju Lu Zhiwei Chen Chenghong Liu 

机构地区：[1]Biotech Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,200000,China [2]Biotechnology Research Institute,Shanghai Key Laboratory of Agricultural Genetics and Breeding,Shanghai,200000,China [3]Shanghai Baixin Biotechnology Company Limited,Shanghai,200000,China

出　　处：《Phyton-International Journal of Experimental Botany》2022年第7期1419-1428,共10页国际实验植物学杂志（英文）

基　　金：Key Technology R&D Project of Shanghai Agriculture-developed with Science&Technology Program,China(Grant No.2018(1-2));the Natural Science Foundation of Shanghai,China(Grant No.19ZR1417000);the National Key Research and Development Program of China(2018YFD1000702-5);the Climbing Plan of Shanghai Academy of Agricultural Sciences(2022);the earmarked fund for China Agriculture Research System of MOF and MARA(CARS-05-01A-02).

摘　　要：Phytohormones play important roles in plant growth and development,and polyploids are thought to be an important method for plant breeding.However,the relationship between ploidy and phytohormone is still unclear.In this study,barley at three ploidy levels were produced by microspore culture.Therefore,we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and distribution.The results showed that Abscisic acid(ABA),gibberellin(GA),jasmonic acid(JA),auxin(IAA),salicylic acid(SA)and cytokinin(CTK)were successfully determined in shoots and roots using LC-MS(liquid chromatography mass spectrometry).By comparing the shoots of the haploid and diploid plants,it was found that the distribution trend of the six phytohormones was consistent,and another consistent trend was found in the roots of the diploid and tetraploid plants.In addition,we further analyzed the shoot/root ratio of the different phytohormones to identify the potential differences for haploid,diploid and tetraploid.Here,the relationship between ploidy and phytohormone we provided would provide new insights into understanding the new phenotypes that occur in polyploid species.

关 键 词：Barley POLYPLOIDY microspore culture PHYTOHORMONE shoot root 

分 类 号：Q94[生物学—植物学]