The Endosperm-Specific Expression of YUCCA Genes Enhances Rice Grain Filling  

作　　者：Huijun Jiang Kaien Zhai Xiaofan Ye Tianwei Hu Jieming Jiang Xiaoqiu Dong Weihuai Pan Jianwei Pan Jianxin Shou 

机构地区：[1]College of Life Sciences,Shaoxing University,Shaoxing,312000,China [2]College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua,321004,China [3]School of Life Sciences,Lanzhou University,Lanzhou,730000,China [4]School of Life Science,South China Normal University,Guangzhou,510631,China

出　　处：《Phyton-International Journal of Experimental Botany》2022年第12期2633-2648,共16页国际实验植物学杂志（英文）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.31801193,31820103008,91754104,and 31670283);the Fundamental Research Funds for the Central Universities(No.lzujbky-2020-it13).

摘　　要：Grain filling is a crucial process that affects yield in rice(Oryza sativa L.).Auxin biosynthesis and signaling are closely related to rice yield;therefore,it is important to understand the effects of auxin biosynthesis on rice grain filling to improve crop yield.In this study,we used physiological and molecular strategies to identify the roles of auxin in rice grain filling.Exogenous application of auxin(IAA)or auxin analogues(2,4-D)to young spikelets and flag leaves improved the seed-setting rate and yield per spike.Furthermore,real-time quantitative PCR assays confirmed that nine members of the OsYUCCA family of auxin biosynthetic genes were upregulated during grain filling,implication that auxin biosynthesis plays a major role in grain development.The specific expression of either Arabidopsis AtYUCCA1 or OsYUCCA2 in the endosperm or leaves resulted in increased expression of OsIAA genes and auxin content of seeds,as well as increased grain filling and seed-setting rate.This result establishes that the auxin content in grains and leaves is important for grain development.Our findings further highlight the potential applications for improving rice yield by elevating targeted gene expression in specific tissues.

关 键 词：Auxin content grain filling IAA biosynthesis rice seed-setting rate YUCCA genes 

分 类 号：Q94[生物学—植物学]