1株发光杆菌Photorhabdus asymbiotica的次级代谢产物研究  

Secondary metabolites from a bacterium of Photorhabdus asymbiotica

作　　者：卢宇 闫璧滢 陈渝川 朱小红 李妍[2] 司书毅[2] 陈明华[2] 袁丽杰 Lu Yu;Yan Bi-ying;Chen Yu-chuan;Zhu Xiao-hong;Li Yan;Si Shu-yi;Chen Ming-hua;Yuan Li-jie(Tangshan Key Laboratory for Preclinical and Basic Research on Chronic Disease,Hebei Key Laboratory for Chronic Diseases,School of Basic Medical Science,North China University of Science and Technology,Tangshan 063210;Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050)

机构地区：[1]华北理工大学基础医学院,河北省慢性疾病基础医学重点实验室,唐山市慢性病临床基础研究重点实验室,唐山063210 [2]中国医学科学院北京协和医学院医药生物技术研究所,北京100050

出　　处：《中国抗生素杂志》2022年第7期677-681,共5页Chinese Journal of Antibiotics

基　　金：中国医学科学院医学与健康科技创新工程经费(No.2021-I2M-1-028);国家自然科学基金(No.81302675);河北省自然科学基金(No.H2021209027)。

摘　　要：目的研究1株发光杆菌Photorhabdus asymbiotica抗革兰阴性菌的活性次级代谢产物。方法以抗革兰阴性菌活性为导向,采用大孔吸附树脂、MCI柱色谱,以及反相高效液相色谱等多种分离纯化技术,利用HR-ESIMS和NMR等波谱学方法,分离Photorhabdus asymbiotica发酵液中的化合物并鉴定其结构。结果从菌株Photorhabdus asymbiotica中获得3个次级代谢产物,并鉴定为darobactin(1)、环(L-色氨酸-L-脯氨酸)(2)和环(L-脯氨酸-L-苯丙氨酸)(3)。其中化合物1对大肠埃希菌、肺炎克雷伯菌和鲍曼不动杆菌均具有显著的抗菌活性。结论化合物1为发光杆菌Photorhabdus asymbiotica中主要的抗革兰阴性菌活性化学成分。Objective To study the secondary metabolites with anti-Gram-negative bacteria activity produced by a bacterium of Photorhabdus asymbiotica.Methods The secondary metabolites were isolated from the fermentation of Photorhabdus asymbiotica by a variety of chromatographic techniques including macroporous resin,MCI gel,and reverse high performance liquid chromatography.Their structures were identified by HR-ESI-MS,NMR spectral analysis,and comparison with the spectral data from literatures.Results Three known compounds were identified as darobactin(1),cyclo(L-Pro-L-Phe)(2),and cyclo(L-Trp-L-Pro)(3).Compound 1 showed significant antibacterial activity against Escherichia coli,Klebsiella pneumoniae,and Acinetobacter baumannii with the MICs values of 1.56~12.5μg/mL.Conclusion Darobactin is the main bioactive component of the bacteria Photorhabdus asymbiotica.

关 键 词：发光杆菌 Darobactin 肽类 抗革兰阴性菌活性 

分 类 号：R978.1[医药卫生—药品]