广州市生活污水中埃可病毒11型循环动态变化及基因特征分析  

Temporal Fluctuation and Genetic Characterization of Echovirus-11 Isolates upon Surveillance of Domestic Sewage in Guangzhou City,China

作　　者：黄淑芬 张薇[2] 陈美钟 郭雪[2] 祝双利[3] 李彩霞 曾汉日[2] 方苓[2] 张勇[3] 郑焕英[2] 邓小玲[2,1] HUANG Shufen;ZHANG Wei;CHEN Meizhong;GUO Xue;ZHU Shuangli;LI Caixia;ZENG Hanri;FANG Ling;ZHANG Yong;ZHENG Huanying;DENG Xiaoling(School of Public Health,Southern Medical University,Guangzhou 510515,China;Guangdong Provincial Center for Disease Control and Prevention,Guangdong Workstation for Emerging Infectious Disease Control and Prevention,Guangzhou 511430,China;WHO WPRO Regional Polio Reference Laboratory,National Health and Family Planning Commission Key Laboratory for Medical Virology,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]南方医科大学公共卫生学院,广州510515 [2]广东省疾病预防控制中心,广东新发突发传染病防治工作站,广州511430 [3]世界卫生组织西太平洋区脊髓灰质炎参比实验室,国家卫生健康委员会生物安全重点实验室,国家卫生健康委员会医学病毒学和病毒病重点实验室,中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《病毒学报》2022年第5期1108-1116,共9页Chinese Journal of Virology

基　　金：国家重点研发计划项目(项目号:2021YFC2302003),题目:病毒监测网络数据标准及数据平台建设。

摘　　要：埃可病毒11型(Echovirus 11,E11)属于B组肠道病毒,是最常见的人类肠道病毒(Enterovirus,EV)之一,是引起无菌性脑膜炎,新生儿感染的重要病原体之一。为探讨广州市生活污水监测中E11分离株循环动态变化及其基因组特征,本研究对2009-2021年广州市生活污水EV监测获得的E11分离株进行VP1区基因扩增及测序,结合2019年广东省发生的两起E11新生儿感染事件及部分散发病例中获得的E11分离株,与全球E11参考株进行序列相似性分析和系统发生学分析。结果显示,2009-2021年生活污水EV监测中E11分离株的核苷酸及氨基酸相似性分别为72.8%~99.3%和84.5%~99.6%。监测发现,广州市2018-2019年在污水中检出大量E11,与两起新生儿感染事件及散发病例的分离株核苷酸相似性高达96.2%~99.2%,但序列间仍存在差异,两起新生儿感染及散发病例感染的毒株为不同来源。通过系统进化树分析发现,2018-2019年污水E11分离株及两起新生儿感染与散发病例E11亲缘关系较近,分子分型结果均为E11 D5基因型。2017年及以前的污水分离株以A1基因型为主,但也有C3和D5基因型,2018年开始则以D5基因型为主,与同时期EV感染病例基因型结果一致。而在2021年污水中则发现了E11 E基因型,但广州暂未有该基因型的感染病例报道,仍需持续进行病例和环境监测。结果表明,生活污水监测能够及时反映EV在环境和人群中的循环动态变化,对EV引起的相关疾病起到预测预警作用。Echovirus 11(E11),classified into enterovirus(EV)group B,is one of the most commonly isolated human EVs.Most lethal EV infections and aseptic meningitis are caused by E11.We planned to analyze the genetic characterization of E11 from samples of domestic sewage in Guangzhou City(China).The VP1 coding region of the strains were amplified.Phylogenetic analyses on the VP1 sequences from these isolates,strains isolated from two E11 neonatal infection events,and some sporadic cases in Guangdong Province in 2019 and others published in GenBank were conducted.From 2009 to 2021,several E11 strains were isolated from samples of domestic sewage in Guangzhou City in Guangdong Province,China.Among these isolates,nucleotide(nt)identities were 72.8%–99.3%,whereas amino acid(aa)identities were 84.5%–99.6%.The nt identities were 96.2%–99.2%between the strains isolated from sewage in 2018–2019 and the strains isolated from two neonatal infection events as well as some sporadic cases in Guangdong Province in 2019,China.The strains mentioned above had the D5 genotype.However,there were differences between the sequences,and the two events and sporadic cases were infected with strains from different sources.From 2009to 2017,the dominant transmission chain of E11 was the A1 genotype,but there were also C3 and D5genotypes.Since 2018,the dominant transmission chain of E11 was the D5 genotype,which was consistent with the genotyping results of the EV infection cases during the same period.Genotype E was found in isolates of domestic sewage in 2021,but no case of infection with this genotype was reported in Guangzhou,China.Continued surveillance of cases and sewage is required.In conclusion,timely surveillance of domestic sewage reflects the circulation of human EVs,which has practical importance for prediction and early warning of human EV-related diseases.

关 键 词：埃可病毒11型 污水监测 基因特征 循环动态变化 

分 类 号：R373.2[医药卫生—病原生物学]