Chemical constituents of Hainan Syzygium myrsinifolium(Hance)Merr.et Perry based on ultra-high performance liquid chromatography-time-of-flight tandem mass spectrometry  

UPLC-Q-TOF-MS/MS分析海南竹叶蒲桃的化学成分

作　　者：Chaohai Pang Yuan Xu Xionghui Ma Yuwei Wu Shuhuai Li Pingping Li Zhenshan Zhang 庞朝海;许园;马雄辉;吴雨薇;黎舒怀;李萍萍;张振山(中国热带农业科学院分析测试中心,海南海口571101;农业农村部亚热带果品蔬菜质量安全控制重点实验室,海南海口571101;海南省热带果蔬产品质量安全重点实验室,海南海口571101;国家市场监管重点实验室(热带果蔬质量与安全,海南海口571101;海南医科大学药学院热带转化医学教育部重点实验室,海南海口571199)

机构地区：[1]Analysis and Test Center,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China [2]Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable,Ministry of Agriculture and Rural Affairs,Haikou571101,China [3]Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables,Haikou 571101,China [4]Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation,Haikou 571101,China [5]Key Laboratory of Tropical Translational Medicine of Ministry of Education,School of Pharmacy,Hainan Medical University,Haikou 571199,China

出　　处：《Journal of Chinese Pharmaceutical Sciences》2022年第7期507-516,共10页中国药学（英文版）

基　　金：Natural Science Foundation of Hainan Province(Grant No.320QN302);the Central Public-interest Scientific Institution Basal Research Fund for the Chinese Academy of Tropical Agricultural Sciences(Grant No.1251632022005);the Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation(Grant No.ZX-2022002);Open Fund of Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables(Grant No.KFKT2021002)。

摘　　要：Syzygium myrsinifolium(Hance)Merr.et Perry is a unique plant resource on Hainan Island,and there are few reports on its chemical constituents.In the present study,we identified the major chemical constituents in the leaves of Syzygium myrsinifolium(Hance)Merr.et Perry using ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS).The chemical constituents in Syzygium myrsinifolium(Hance)Merr.et Perry were identified rapidly based on the accurate relative molecular mass and combined with literature data and reference substances.A total of 20 compounds,including organic acids,phenolic acids,and flavonoids,were identified.Among these 20 compounds,four organic acids,eight phenolic acids,seven flavonoids,and one coumarin were reported from the leaves of Syzygium myrsinifolium(Hance)Merr.et Perry for the first time.The established method was rapid and accurate,and some chemical constituents in the leaves of Syzygium myrsinifolium(Hance)Merr.et Perry have been identified.This research provided an experimental reference for the study of the constituents and utilization of Syzygium myrsinifolium(Hance)Merr.et Perry.竹叶蒲桃是海南岛特有的植物资源,目前关于它化学成分的研究报道很少。本研究采用超高效液相色谱-四极杆-飞行时间串联质谱联用技术(UPLC-Q-TOF-MS/MS)鉴定竹叶蒲桃叶片中的主要化学成分。通过高分辨质谱数据分析、参考文献数据及对照品确认,对竹叶蒲桃的化学成分进行快速的定性分析,鉴定并推断出包括有机酸、酚酸、黄酮和香豆素在内的20种化合物,并对其裂解规律进行分析。其中4个有机酸、8个酚酸、7个黄酮和1个香豆素类化合物首次在竹叶蒲桃叶片中报道。本研究建立的方法能快速、准确地对竹叶蒲桃叶片中部分成分进行鉴定解析,为竹叶蒲桃植物资源的成分研究和开发利用提供了实验依据。

关 键 词：Syzygium myrsinifolium(Hance)Merr.et Perry UPLC-Q-TOF-MS/MS Identification 

分 类 号：R284[医药卫生—中药学]