补阳还五汤调节血管内皮细胞炎症信号通路干预高血压前期血管损伤机制  

Mechanism of Buyang Huanwu Decoction Regulating Inflammatory Signal Pathway of Vascular Endothelial Cells and Intervening Vascular Injury in Prehypertension

作　　者：薛亚楠[1,2] 张立德[2] 王建波 孙杰[1] 曲怡 XUE Ya-nan;ZHANG Li-de;WANG Jian-bo;SUN Jie;QU Yi(Liaoning University of Traditional Chinese Medicine,Innovative Engineering Technology Center of Traditional Chinese Medicine,Shenyang 110000,China;Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications,Shenyang 110000,China)

机构地区：[1]辽宁中医药大学中医药创新工程技术中心,沈阳110000 [2]中医脏象理论及应用教育部重点实验室,沈阳110000

出　　处：《科学技术与工程》2022年第25期10906-10911,共6页Science Technology and Engineering

基　　金：辽宁省百千万人才工程项目(2020921097);辽宁省教育厅育苗项目(L202025);国家自然科学基金(81302880);国家重点基础研究发展计划(973计划)(2012CB518503)。

摘　　要：观察补阳还五汤调控炎症信号通路对肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)诱导的人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)损伤的影响。采用10 ng/mL TNF-α建立细胞损伤模型,使用不同浓度的补阳还五汤(0.5%、1%、2%)干预处理24 h后收集细胞。通过Hoechst 33342染色高内涵观测细胞数量,Western blot检测炎症信号通路NF-κB p65和IκB的蛋白表达水平,逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)检测相关炎症因子NF-κB、IκB、ICAM-1、VCAM-1、MCP-1的mRNA表达。结果表明,应用10 ng/mL TNF-α预处理HUVECs 24 h可明显降低细胞存活率,细胞数量明显减少,NF-κB p65蛋白表达升高,IκB蛋白和mRNA表达降低,NF-κB、ICAM-1、VCAM-1、MCP-1 mRNA表达水平增加(P<0.05)。补阳还五汤干预处理后,细胞数量明显增加,且抑制了NF-κB p65及炎症因子NF-κB、ICAM-1、VCAM-1、MCP-1的高表达,IκB蛋白和mRNA表达水平均显著下降(P<0.05)。补阳还五汤通过抑制NF-κB炎症信号通路的激活,降低了炎症因子的高表达,对TNF-α诱导的HUVECs损伤起到保护作用。To observe the effect of Buyang Huanwu Decoction on regulating inflammatory signal pathway to the injury of human umbilical vein endothelial cells(HUVECs)which induced by tumor necrosis factor-α(TNF-α).The cell injury model was established by TNF-α(10 ng/mL).Cells were collected 24 h after intervention with different concentrations of Buyang Huanwu Decoction(0.5%,1%,2%).The number of cells was observed by Hoechst 33342 staining,the inflammatory signal pathway factor of NF-κB p65 protein and IκB protein was detected by Western blot,the mRNA contents of inflammatory factor NF-κB,IκB,ICAM-1,VCAM-1 and MCP-1 was detected by reverse transcription-polymerase chain reaction(RT-PCR).The results show that the number of cells is significantly reduced,the expression of NF-κB p65 protein and the mRNA expression levels of NF-κB,ICAM-1,VCAM-1 and MCP-1 are apparently increased,NF-κB p65 protein expression increases,IκB protein and mRNA expression both decreases after 24 h by 10 ng/mL TNF-αpretreatment(P<0.05).Buyang Huanwu Decoction can significantly increase the number and activity of cells,inhibit the high expression of NF-κB p65 protein and the mRNA expression levels of NF-κB,ICAM-1,VCAM-1 and MCP-1,the protein and mRNA expression levels of IκB are increased significantly(P<0.05).By inhibiting the activation of NF-κB inflammatory signaling pathway,Buyang Huanwu Decoction reduces the high expression of inflammatory factors and protects HUVECs injury induced by TNF-α.

关 键 词：补阳还五汤 内皮细胞 炎症 高血压 

分 类 号：R284.2[医药卫生—中药学]