蜡蚧轮枝菌JMC-01粗毒素对桃蚜和小菜蛾4种酶活的影响  

Effects of crude toxin of Lecanicillium lecanii JMC-01 on the activities of four enzymes in Myzus persicae and Plutella xylostella

作　　者：柴军发 赵雪怡 张暄翊 洪波[1] 贾彦霞[1] CHAI Jun-fa;ZHAO Xue-yi;ZHANG Xuan-yi;HONG Bo;JIA Yan-xia(School of Agriculture,Ningxia University,Yinchuan750021,China)

机构地区：[1]宁夏大学农学院,银川750021

出　　处：《生态学杂志》2022年第9期1726-1730,共5页Chinese Journal of Ecology

基　　金：宁夏回族自治区农业科技自主创新资金“十四五”农业高质量发展和生态保护科技创新示范项目(NGSB-2021-10-04)资助。

摘　　要：为了开发蜡蚧轮枝菌的新型制剂防治蔬菜害虫,本实验利用乙酸乙酯提取蜡蚧轮枝菌JMC-01发酵8 d后的粗毒素,以无翅桃蚜和小菜蛾3龄幼虫为试虫,测定了粗毒素对试虫的室内毒力及处理后试虫体内解毒酶(谷胱甘肽硫转移酶(GST),羧酸酯酶(CarE)),乙酰胆碱酯酶(AchE)和过氧化物酶(POD)的活性变化。结果表明:JMC-01粗毒素对桃蚜和小菜蛾的毒力分别高达94.25%和85.41%,LC_(50)为37.62和43.42 mg·L^(-1);处理蚜虫后,CarE活力为0.81 U·mg^(-1) prot, AchE活力为0.1173 U·mg^(-1) prot, GST活力为22.52 U·mg^(-1) prot, POD活性为5.29 U·mg^(-1) prot;处理小菜蛾后,CarE、AchE、GST、POD活性分别达0.85、0.1176、27.5和13.25 U·mg^(-1) prot。本试验通过研究蜡蚧轮枝菌JMC-01粗毒素对桃蚜和小菜蛾的毒力及体内解毒酶和保护酶活性变化,进一步验证了粗毒素中含有天然的杀虫活性物质,为真菌生防制剂的进一步开发提供思路。In order to develop a new agent of Lecanicillium lecanii to control pests on vegetable, the crude toxin of Lecanicillium lecanii JMC-01 fermented for eight days was extracted by ethyl acetate, with which the apterous Myzus persicae and 3 rd instar larvae of diamondback moth(Plutella xylostella) were treated. We measured the indoor virulence of the test insects and the changes of glutathione S-transferase(GST), carboxylesterase(CarE), acetyl cholinesterase(AchE) and peroxidase(POD) activities of the test insects. The results showed that the toxicity of JMC-01 crude toxin to M. persicae and P. xylostella reached 94.25% and 85.41%, and the LCvalues were 37.62 and 43.42 mg·L^(-1), respectively. After being treated with JMC-01 crude toxin, the activities of CarE, AchE, GST, and POD in M. persicae increased to 0.81, 0.1173, 22.52, and 5.29 U·mg^(-1)prot, respectively, while those of P. xylostella increased to 0.85, 0.1176, 27.5, and 13.25 U·mg^(-1)prot, respectively. Our results demonstrated that the JMC-01 crude toxin contained natural insecticidal active substances by examining the virulence of the crude toxin to M. persicae and P. xylostella, as well as the changes of detoxification enzyme and protective enzyme activities, which provided basis for the further development of fungal biocontrol agents.

关 键 词：蜡蚧轮枝菌 粗毒素 致病性 酶活 

分 类 号：S476.12[农业科学—农业昆虫与害虫防治]