苦草生长对溱湖沉积物磷含量及细菌群落结构的影响  

Dynamics of phosphorus concentration and bacterial community structure in sediment of Qinhu Lake as influenced by Vallisneria natans

作　　者：王娟娟 谢涛 阙天洋 张思文 钱晓晴[1] 吕世鹏 WANG Juan-juan;XIE Tao;QUE Tian-yang;ZHANG Si-wen;QIAN Xiao-qing;LYU Shi-peng(College of Environmental Sciences and Engineering,Yangzhou University,Yangzhou 225127,Jiangsu,China;Institute of Botany,Jiangsu Province and Chinese Academy of Sciences,Nanjing 210014,China)

机构地区：[1]扬州大学环境科学与工程学院,江苏扬州225127 [2]江苏省中国科学院植物研究所,南京210014

出　　处：《生态学杂志》2022年第9期1787-1795,共9页Chinese Journal of Ecology

基　　金：国家自然科学基金项目(41701093);江苏省研究生科研与实践创新计划项目(KYCX19_2110);中国博士后基金(017M611928)资助。

摘　　要：恢复沉水植物生长是富营养化水体修复的重要手段。为了探究苦草生长条件下泰州溱湖沉积物磷含量及细菌群落结构的季节性变化,基于16S rRNA基因高通量测序技术,分析不同时期(1、5和9月)苦草生长水域与对照水域沉积物中细菌多样性及群落结构的差异,并探究主要环境因子的变化规律。结果表明,苦草生长降低了溱湖沉积物总磷与有效氮含量,提高了生物有效性铁、有机质含量与铁磷占比。苦草与对照区沉积物主要优势细菌门为变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、酸杆菌门(Acidobacteria)、放线菌门(Actinobacteriota)与脱硫菌门(Desulfobacterota)。细菌群落结构有显著季节性变化,苦草生长区沉积物变形菌门和脱硫菌门的相对丰度有所增加,而绿弯菌门和酸杆菌门相对丰度则呈下降趋势。冗余分析表明,湖水pH与沉积物总磷对细菌群落的空间分布影响最大,其次是溶解氧含量。研究表明,苦草生长可以通过改变溱湖湖水及沉积物理化性质影响沉积物细菌群落组成,从而增加磷固定并改善湖泊水质。Restoring submerged macrophytes is of significant importance for the remediation of water eutrophication. Sediment samples with or without Vallisneria natans were collected at Qinhu Lake, Taizhou in January, May and September of 2019. We analyzed phosphorus content and examined bacterial community structure in sediments by high-throughput sequencing of the 16 S rRNA gene. The environmental factors were also investigated. The results showed that the growth of V. natans reduced the concentrations of total phosphorus(TP) and available nitrogen contents in sediments, but increased bioavailable iron, organic matter contents and Fe-P/TP ratio. The dominant bacterial phyla in the sediments included Proteobacteria, Chloroflexi, Acidobacteria, Actinobacteriota and Desulfobacterota, with seasaonal variations of bacterial community structure. With the presence of V. natans, the relative abundance of Proteobacteria and Desulfobacterota increased and the relative abundance of Chloroflexi and Acidobacteria decreased. Results of the redundancy analysis showed that water pH and TP in sediments had the most significant influence on the spatial distribution of bacterial community, followed by dissolved oxygen. Overall, our results indicated that the growth of V. natans affected bacterial community composition in sediments by changing the physical and chemical properties of lake water and sediments, and thus increased phosphorus fixation efficiency in sediments and improved water quality of the lake.

关 键 词：苦草 沉积物 磷 细菌群落结构 

分 类 号：X524[环境科学与工程—环境工程] X17