290个吉林省审定玉米品种SSR-DNA指纹构建及遗传多样性分析  

Construction of SSR-DNA fingerprints and genetic diversity analysis of 290 maize varieties approved in Jilin province,China

作　　者：田红丽[1] 赵紫薇 杨扬[1] 范亚明 班秀丽[3] 易红梅[1] 杨洪明[3] 刘少荣 高玉倩 刘亚维[1] 王凤格[1] TIAN Hong-Li;ZHAO Zi-Wei;YANG Yang;FAN Ya-Ming;BAN Xiu-Li;YI Hong-Mei;YANG Hong-Ming;LIU Shao-Rong;GAO Yu-Qian;LIU Ya-Wei;WANG Feng-Ge(Maize Research Center,Beijing Academy of Agriculture and Forestry Sciences/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecu-lar Breeding,Beijing 100097,China;Jilin Agricultural University,Changchun 130118,Jilin,China;Jilin Seed Management Station,Changchun 130022,Jilin,China)

机构地区：[1]北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室,北京100097 [2]吉林农业大学,吉林长春130118 [3]吉林省种子管理总站,吉林长春130022

出　　处：《作物学报》2022年第12期2994-3003,共10页Acta Agronomica Sinica

基　　金：北京市农林科学院科技创新能力建设专项(KJCX20200305);“十三五”国家重点研发计划项目(2017YFD0102001)资助。

摘　　要：吉林省审定玉米品种标准指纹构建及遗传多样性分析,可为其品种审定、保护、市场监测等提供核心数据支撑,同时对于品种选育、种质创新也具有重要意义。本研究利用40对SSR标记构建了吉林省2010—2017年审定的290个玉米品种标准SSR-DNA指纹并进行了遗传多样性分析。结果表明,40对SSR标记检测到等位基因数平均值为11.85个,PIC平均值为0.64;品种杂合基因型频率平均值为0.66;品种间差异位点数目均≥2个。6个熟期组的遗传多样性分析显示,中晚熟和中熟审定的品种较多占71.72%,极早熟、早熟、晚熟审定品种较少;早熟组具有基因多样性参数最高值0.69,品种数量最多的中晚熟组具有相对低的基因多样性值。聚类结果显示,吉林省审定推广种植品种被划分为3类,分别是甜糯爆裂、极早熟和早熟类、偏中或晚熟类;偏中或晚熟的品种显示了两大趋势,代表了选用杂优群的差异、品种选育单位的不同、品种类型的差异等。本研究为吉林省玉米品种管理、品种选育、种质创新提供了重要的理论和数据基础。The standard fingerprint construction and genetic diversity analysis of maize varieties approved in Jilin province can provide data support for variety approval,protection,and market monitoring.It is also of great significance for variety breeding and germplasm innovation.In this study,40 pairs of SSR markers were used to construct the standard SSR-DNA fingerprints of 290 maize varieties approved in Jilin province from 2010 to 2017,and the genetic diversity was analyzed.The results showed that the average number of alleles detected by 40 SSRs was 11.85 and the average PIC was 0.64.The average frequency of heterozygous genotypes of varieties was 0.66.The different loci number among varieties was≥2.The genetic diversity analysis of six maturity groups revealed that there were more varieties with middle and late maturity,accounting for 71.72%,and fewer varieties with extremely early maturity,early maturity,and late maturity.The early maturity group had the highest gene diversity parameter of 0.69,and the mid-late maturity group with the largest number of varieties had a relatively low gene diversity value.Clustering results indicated that the varieties approved and popularized in Jilin province were divided into three categories:sweet waxy and popcorn,extremely early and early middle,medium or late maturity.The middle or late maturing varieties had two major trends,representing the differences in the selection of heterozygous groups,the different breeding units,the differences in representing the differences in the selection of heterozygous groups,the different breeding units,the differences in variety types and so on.This study provides an important theoretical and data basis for maize variety management,variety breeding,and germplasm innovation in Jilin province.

关 键 词：吉林省 审定玉米品种 SSR标记 DNA指纹构建 遗传多样性 

分 类 号：S513[农业科学—作物学]