迷迭香酸对增强交联透明质酸透皮吸收的作用  

Effect of Rosmarinic Acid(RosA)on Enhancing Transdermal Absorption of Crosslinked Hyaluronic Acid(CHA)

作　　者：刘杰[1] 何浩明 周晓鹰[1] LIU Jie;HE Haoming;ZHOU Xiaoying(School of Pharmacy,Changzhou University,Changzhou 213164,China;Changzhou Pharmaceutical Research Institute,Changzhou 213164,China)

机构地区：[1]常州大学药学院,江苏常州213164 [2]常州药物研究所,江苏常州213164

出　　处：《常州大学学报（自然科学版）》2022年第5期72-81,共10页Journal of Changzhou University：Natural Science Edition

基　　金：海外人才研究实验室启动资助项目(Z391405);江苏省研究生研究与实践创新资助项目(KYCX19_1777);常州大学生命科学研究资助项目(ZMF14020066)。

摘　　要：研究迷迭香酸(RosA)对增强交联透明质酸(CHA)透皮吸收的影响,获取RosA并制备CHA。建立体外大鼠透皮分析模型,通过微量CHA检测方法和细胞毒性测定模型,研究透明质酸和交联透明质酸在不同时间和温度下的稳定性,评估RosA对CHA透皮的增强作用,并与氮酮比较,同时考察RosA的细胞毒性。结果显示含体积分数5%RosA的CHA皮肤渗透增强,比常规氮酮有效。随着RosA质量浓度的增加,交联透明质酸的透皮率随之增加(***P=0.001)。RosA不显示体外细胞毒性,用于交联透明质酸渗透促进剂的RosA(10 mg/mL)不影响细胞生长。To investigate the effects of Rosmarinic acid(RosA)on enhancing the transdermal absorption of cross-linked hyaluronic acid(CHA),RosA was obtained and CHA was prepared.The rat-skin transdermal in vitro model was established,the CHA micro-assay and cytotoxicity model were established.The stability of HA and CHA at different time-points and temperatures were investigated,the enhancing effects of RosA and combinations on CHA transdermal were evaluated,and the cytotoxicity of RosA were investigated.The results showed that 5%RosA enhanced skin penetration of CHA and was more effective than conventional azone.With the increase of RosA concentrations,the transdermal rate of CHA was increased(***P=0.001).RosA(10 mg/mL)used as the CHA transdermal penetration enhancer did not affect cell growth in vitro.

关 键 词：迷迭香酸 交联透明质酸 透皮吸收 天然渗透促进剂 细胞毒性 

分 类 号：R9[医药卫生—药学]