新风胶囊对佐剂关节炎大鼠外周血巨噬细胞CD86、CD206表达影响  

Effect of Xinfeng capsule on CD86, CD206 expression in peripheral blood macrophages in rats with adjuvant arthritis

作　　者：马熙檬 万磊[2] 刘健[2] 黄传兵[2] 赵磊 孙广瀚 朱子衡 李舒 李方泽 刘天阳[2] 刘磊[2] 李明[2] MA Xi-meng;WAN Lei;LIU Jian;HUANG Chuan-bing;ZHAO Lei;SUN Guang-han;ZHU Zi-heng;LI Shu;LI Fangze;LIU Tian-yang;LIU Lei;LI Ming(Graduate Department of Anhui University of Traditional Chinese Medicine,Hefei 230038,Anhui;Department of Rheumatology,the First Afiliated Hospital of Anhui University of Traditional Chinese Medicine,Hefei 230031,Anhui)

机构地区：[1]安徽中医药大学研究生院,安徽合肥230038 [2]安徽中医药大学第一附属医院风湿科,安徽合肥230031

出　　处：《中药与临床》2022年第2期58-61,共4页Pharmacy and Clinics of Chinese Materia Medica

基　　金：国家自然基金面上项目(81973655);安徽省高校自然科学研究项目(KJ2020A0397);科技部国家重点研发计划中医药现代化研究重点专项(2018YFC1705204);全国中医药创新骨干人才培训项目(国中医药人教函[2019]128号);安徽中医药大学校基金项目(2018xrxd17);安徽中医药大学第一附属医院院内基金(2020yfyzc25、2020yfyzc55)项目资助。

摘　　要：目的:探讨新风胶囊对佐剂性关节炎大鼠(AA)外周血巨噬细胞CD86、CD206表达之间的关联。方法:将30只大鼠随机分为正常(NC)组、模型(MC)组和新风胶囊组(XFC),每组10只,向模型组、新风胶囊组动物右后足跖皮内注射弗氏完全佐剂(0.1 mL/只)以致炎。致炎后第12天,观察各组大鼠足跖肿胀度(E)的变化,流式细胞术检测大鼠外周血炎症极化标志物CD68、CD206的表达。结果:与正常组相比,模型组大鼠E、CD68表达明显升高(P<0.01或P<0.05);CD206表达降低(P<0.01或P <0.05)。相关性结果显示,足趾肿胀度与CD206呈负相关(P <0.05),足趾肿胀度与CD68呈正相关(P <0.05或P <0.01);XFC与足趾肿胀度、CD68与呈负相关,与CD206呈正相关(P<0.05或P <0.01)。结论:魔新风胶囊可能通过调控巨噬细胞CD68、CD206炎症极化,抑制免疫炎症反应,改善佐剂性关节炎AA(病情活动性、关节破坏及预后)。Objective: To explore the influence of Xinfeng capsule on CD86 and CD206 expression in peripheral blood macrophages in rats with adjuvant arthritis(AA). Method: 30 rats were randomly divided into normal(NC), model(MC) and Xinfeng capsule(XFC) groups with 10 rats in each group. The rats in MC and XFC groups were injected intradermally in the right hind plantar crease with Freund’s complete adjuvant(0.1 ml/rat) to cause inflammation. On the 12th day after inflammation, the plantar swelling degree(E) of rats in each group was observed, and the expression of inflammatory polarization markers CD68and CD206 in peripheral blood of rats were detected by flow cytometry. Result: Compared with NC group, the E value and CD68expression in rats of MC group were significantly increased(P < 0.01 or P < 0.05), and CD206 expression was decreased(P < 0.01or P < 0.05). Correlation results showed that E value was negatively correlated with CD206(P <0.05), and positively correlated with CD68(P < 0.05 or P < 0.01). XFC was negatively correlated with E value and CD68, and positively correlated with CD206(P < 0.05 or P < 0.01). Conclusion: XFC may inhibit immune inflammation by regulating inflammatory polarization-related CD68and CD206, thus improving AA(disease activity, joint destruction and prognosis).

关 键 词：佐剂关节炎 炎症极化 CD86、CD206 巨噬细胞 

分 类 号：R285.5[医药卫生—中药学]