基于双波长高效液相色谱法同时测定九蒸九晒熟地黄中7种成分的含量  

Simultaneous determination of seven constituents in the processing of nine steamed and nine sundried Rehmanniae radix by dual wavelength HPLC

作　　者：徐廉松 牛雯雯 孔雪 邢亚东 李娴 XU Liansong;NIU Wenwen;KONG Xue;XING Yadong;LI Xian(School of Pharmacy,Bengbu Medical College,Bengbu,Anhui,233030,China;Pharmaceutical Preparation Section,the First Affliated Hospital of Bengbu Medical College,Bengbu,Anhui,233030,China;Postdoctoral Workstation of Anhui Xiehecheng Pharmaceutical Decocting Pieces Co.Ltd.,Bozhou,Anhui,236800,China;Anhui Provincial Key Laboratory of New Technology of Chinese Materia Medica Decocting Pieces Manufacturing,Bozhou,Anhui,236800,China)

机构地区：[1]蚌埠医学院药学院,安徽蚌埠233030 [2]蚌埠医学院第一附属医院药剂科,安徽蚌埠233030 [3]安徽协和成药业饮片有限公司博士后工作站,安徽亳州236800 [4]中药饮片制造新技术安徽省重点实验室,安徽亳州236800

出　　处：《甘肃中医药大学学报》2022年第4期1-6,共6页Journal of Gansu University of Chinese Medicine

基　　金：国家自然科学基金面上项目(81372899);安徽省高校自然科学研究重点项目(KJ2018A0983);蚌埠医学院横向课题(2019-BYHX-03)。

摘　　要：目的 建立同时测定九蒸九晒熟地黄中梓醇、益母草苷、地黄苷A、地黄苷D、毛蕊花糖苷、异类叶升麻苷、5-羟甲基糠醛(5-HMF)等7种成分含量的方法。方法 采用超声法提取九蒸九晒熟地黄炮制过程中每一次蒸晒后的样品,然后应用高效液相色谱(HPLC)法同时测定样品中7种成分的含量;色谱柱为赛默飞C18(250.0 mm×4.6 mm,5μm),流动相为0.1%磷酸(A)-乙腈(B),梯度洗脱,柱温30℃,检测波长203,332 nm,进样量10μL。结果 7种成分在各自线性范围内线性关系良好,平均加样回收率为97.57%~100.58%,RSD为0.60%~1.78%。结论 该方法准确、稳定,重复性好,可用于九蒸九晒熟地黄饮片的质量控制。Objective To establish a method of simultaneous content determination for 7 constituents including catalpol,leonuride,rehmannioside A,rehmannioside D,acteoside,isoacteoside,and 5-hydroxymethylfurfural( 5-HMF) in the processing of nine steamed and nine sundried Rehmanniae radix. Methods Ultrasonic method was used to extract the samples after each steamed and sundried during the processing of nine steamed and nine sundried Rehmanniae radix. Then,the content determination of 7 constituents in the samples was carried out on simultaneously by high performance liquid chromatography( HPLC),and the chromatographic column was Thermo Fisher C18( 250.0 mm×4.6 mm,5 μm). The mobile phase consisted of 0.1% phosphoric acid( A) and acetonitrile( B) with gradient elution,at the detection wavelength of 203,332 nm. The column temperature was set at 30 ℃,and the injection volume was 10 μL. Results Seven constituents showed good linear relationships within their own ranges. The average recoveries were between 97.67% and 100.46%,with the RSD between 0.58% and 1.76%.Conclusion The method is accurate,stable,repeatable and can be used for quality control of nine steamed and nine sundried Rehmanniae radix in decocting pieces.

关 键 词：九蒸九晒熟地黄 梓醇 益母草苷 地黄苷A 地黄苷D 毛蕊花糖苷 异类叶升麻苷 5-羟甲基糠醛 高效液相色谱 含量测定 

分 类 号：R284.2[医药卫生—中药学]