水稻粒长新基因GL12-1定位与利用分析  

Genetic Identification and Utilization of a Novel Rice Grain Length Gene GL12-1

作　　者：何旎清 黄凤凰[1] 余敏祥 杨德卫[1] HE Niqing;HUANG Fenghuang;YU Minxiang;YANG Dewei(Institute of Rice,Fujian Academy of Agricultural Sciences,Fuzhou 350018,China)

机构地区：[1]福建省农业科学院水稻研究所,福州350018

出　　处：《西北植物学报》2022年第8期1301-1306,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：福建省自然科学基金(2021J01471);福建省属公益项目(2020R1023003);福建省科技重大专项(2020NZ08016);农业高质量发展超越“5511”协同创新工程(XTCXGC2021001);农业高质量发展超越“5511”协同创新工程(XTCXGC2021019);福建省农业科学院青年科技创新团队(CXTD2021001);福建省农业科学院科技创新团队(CXTD2021005-3)。

摘　　要：水稻的粒长是水稻粒型构成的因素之一,直接影响水稻的单产,而粒长又是稻米品质的重要指标之一。该研究利用选育含有稻瘟病抗性基因Pigm-1的长粒恢复系R20-4为研究材料,通过图位克隆的方法,对粒长基因进行鉴定,并对该长粒性状在育种中的应用进行了分析。结果显示:(1)该长粒为显性基因控制的性状。(2)将粒长基因GL12-1初步定位在水稻第12染色体上Indel-12-3和Indel-12-7之间,物理距离约4.5 Mb。(3)通过进一步开发标记,最终将该粒长基因GL12-1定位在标记Indel-10和Indel-16之间,物理距离约900 kb。(4)长粒恢复系R20-4与‘庆源A’、‘定源A’和‘启源A’测交组合的粒长均表现R20-4表型,而与‘靓香A’测交组合的粒长比R20-4更长。研究表明,粒长基因GL12-1为显性基因控制的性状,可能为一个新的粒长控制基因,该研究为后期GL12-1基因的克隆、功能研究以及粒型分子育种奠定了基础。Grain length is one of the components of rice grain shape that directly affects grain yield. Besides, grain length is one of the important indicators of rice quality. In this study, the long-grain restorer line R20-4 containing the blast resistance gene Pigm-1 was used for genetic analysis of grain length. Through map-based cloning, the long-grain gene was identified and its application in breeding was analyzed through analyzing cross combinations. The results showed that:(1) the GL12-1(grain length 12-1) locus is under the genetic control of a dominant Mendelian factor.(2) Through map-based cloning, GL12-1 was initially located to a chromosomal region of about 4.5 Mb between Inde-12-3 and Inde-12-7 on chromosome 12 of rice.(3) Further genetic analysis delimits the GL12-1 locus to a chromosomal region of 900 kb between markers Inde-10 and Inde-16.(4) Fhybrids from the test crosses with ‘Dingyuan A’, ‘Qiyuan A’ and ‘Qingyuan A’ showed the long-grain phenotype of the R20-4 line, whereas Fhybrids from the test cross with ‘Liangxiang A’ showed the grain length phenotype of the ‘Liangxiang A’ that is longer than that of the R20-4 line. The research showed that the GL12-1 locus is under the genetic control of a dominant Mendelian factor, which may be a new gene controlling grain length. Moreover, this study provides a foundation for cloning, characterization and functional analysis which are essential for molecular breeding of the grain type gene GL12-1.

关 键 词：水稻 长粒 GL12-1 基因定位 育种利用 

分 类 号：Q343.17[生物学—遗传学] S511[农业科学—作物学]