油茶谷氧还蛋白编码基因CoROXY19的分离鉴定及功能分析  

Isolation and functional characterization of Camellia oleifera glutaredoxin gene CoROXY19

作　　者：梁心仪 严冰冰 林增 陈嘉丽 李宁 禹佩瑶 汤阳灿 雒建军 黄黎君 LIANG Xinyi;YAN Bingbing;LIN Zeng;CHEN Jiali;LI Ning;YU Peiyao;TANG Yangcan;LUO Jianjun;HUANG Lijun(College of Forestry,Key Laboratory of Cultivation and Protection for Non-wood Forest Trees,Central South University of Forestry and Technology,Changsha 410004,China)

机构地区：[1]中南林业科技大学林学院,经济林培育与保护教育部重点实验室,长沙410004

出　　处：《植物生理学报》2022年第8期1465-1474,共10页Plant Physiology Journal

基　　金：长沙市杰出创新青年培养计划项目(kq2009016);湖南省自然科学基金(2020JJ5970和2021JJ31141);湖南省研究生科研创新项目(CX20210885);湖南省大学生创新创业训练计划项目(S202010538004)。

摘　　要：油茶是一种潜在的经济林模式树种,其研究受到越来越多的重视。以油茶主栽品种‘华硕’为研究材料,通过RT-PCR克隆方法,获得一个高等植物特异的CC型谷氧还蛋白(glutaredoxin,GRX)基因家族成员,命名为Co ROXY19。Co ROXY19基因的开放阅读框(ORF)为444 bp,编码147个氨基酸,分子质量为15.62 kDa,具有典型的谷氧还蛋白结构域,包括酶活性中心、谷胱甘肽结合位点和转录因子结合位点。进化树分析发现CoROXY19与茶树CsROXY19同源关系最近,亚细胞定位发现CoROXY19蛋白在细胞质和细胞核中均有分布,酵母双杂交和双分子荧光互补实验证实CoROXY19与转录因子AtTGA2相互作用,实时荧光定量PCR表明CoROXY19基因在叶片中的表达受机械损伤诱导,预示油茶CoROXY19可能通过与转录因子相互作用,参与调节抗逆相关激素信号转导。As a potential model tree species,Camellia oleifera has attracted increasing attention from the non-timber forest researchers.A higher plant-specific CC-type glutaredoxin(GRX) gene family member named CoROXY19 was isolated from a cultivar ‘Huashuo’.The open reading frame(ORF) of CoROXY19 is 444 bp,encoding 147 amino acids,and has a molecular mass of 15.62 kDa.It contains several typical glutaredoxin motifs,including an enzyme active center,glutathione binding site and transcriptional protein binding sites.Phylogenetic tree analysis showed that CoROXY19 has a close homology relationship with CsROXY19 from tea plant.Subcellular localization revealed that CoROXY19 protein were distributed in both cytoplasm and nucleus.The two-hybrid experiment confirmed that CoROXY19 interacted with AtTGA2.Real-time quantitative PCR showed that the expression of CoROXY19 was strongly induced by wounding stress.These experimental results suggest that CoROXY19 may be involved in regulating stress-related hormone signal transduction through interacting with transcription factors.

关 键 词：油茶 经济林 谷氧还蛋白 抗逆激素 

分 类 号：S794.4[农业科学—林木遗传育种]