桉树多代连栽引起的土壤细菌群落结构及共现性网络特征变化  

Soil Bacterial Community Structure and Co-occurrence NetworksUnderSuccessive Rotations of PlantationEucalyptus

作　　者：许宇星[1] 王志超[1] 竹万宽[1] 杜阿朋[1] XU Yuxing;WANG Zhichao;ZHU Wankua;DU Apeng(Research Institute of Fast-growing Trees,Chinese Academy of Forestry,Zhanjiang 524022,Guangdong,China)

机构地区：[1]中国林业科学研究院速生树木研究所,广东湛江524022

出　　处：《桉树科技》2022年第3期1-8,共8页Eucalypt Science & Technology

基　　金：中央级公益性科研院所基本科研业务费专项资金《土壤微生物对连栽桉树林改造措施的响应与机理》(CAFYBB2022MA006);广东省林业科技创新项目《雷州半岛多功能人工林构建技术及综合效益评价》(2018KJCX014);广东林业生态监测网络平台建设项目及广东省湛江桉树森林生态系统国家定位观测研究站运营项目。

摘　　要：随着桉树人工林在中国的快速发展,不合理经营措施导致的林地土壤肥力质量下降问题受到种植农户、科研群体和社会大众的高度关注。本研究应用IlluminaMiseq PE300测序平台及共现性网络分析方法,对不同连栽代次桉树人工林土壤细菌网络特征、群落多样性及群落结构组成及其环境驱动因子展开研究。结果表明:尽管多代连栽模式对不同代次林分土壤细菌Alpha多样性未产生显著影响(除细菌丰富度),但集约化经营引起细菌群落结构的显著变化,细菌共现性网络的复杂性和凝聚性均随代次增加明显下降,多代连栽模式引起Acidobacteria丰度显著增加,而Actinobacteria和Patescibacteria的丰度显著下降。土壤硝态氮、有机质含量和容重作为关键环境因子驱动土壤细菌群落结构的演变过程。With the rapid development of the Eucalyptus industry in China,perceptions of declining soil fertility and quality in forest lands due to unreasonable management practices have attracted much attention from plantation growers,research groups and the general public.In this study,Illumina'sMiseq PE300 sequencing platform and co-occurrence network analyses were used to characterize the soil bacterial network,community structure composition and environmental drivers of Eucalyptus plantation over successive rotations.The results showed that although multiple rotations on the one site did not significantly affect soil bacterial Alpha diversity(except for bacterial richness)across rotations,intensive management caused significant changes in bacterial community structure.Also,both the complexity and cohesiveness of bacterial co-occurrence networks decreased remarkably as the number of rotations increased.Meanwhile,multiple successive rotations caused a significant increase in the abundance of Acidobacteria,while the abundance of Actinobacteria and Patescibacteria decreased significantly.It was also found that soil content of nitrate-nitrogen and organic matter along with soil bulk density are the key environmental factors driving the development the soil bacterial community structure.

关 键 词：连栽代次 共现性网络 土壤细菌 微生物群落结构及多样性 

分 类 号：S718.521.3[农业科学—林学]