木麻黄离体器官培养与植株再生的试验研究初报  

Preliminary Study on in vitro Organ Culture and Plant Regeneration of Casuarina equisetifolia

作　　者：容世清 罗建华 李天卓 曾少玲 薛晶晶 全吉文 容卫冰 RONG Shiqing;LUO Jianhua;LI Tianzhuo;ZENG Shaoling;XUE Jingjing;QUAN Jiwen;RONG Weibing(Zhanjiang Forestry Research Institute,Zhanjiang 524037,Guangdong,China)

机构地区：[1]湛江市林业科学研究所,广东湛江524037

出　　处：《桉树科技》2022年第3期53-56,共4页Eucalypt Science & Technology

基　　金：《木麻黄抗青枯病种质资源挖掘创新技术研究》(108000082-2021-0000134138)。

摘　　要：为探索木麻黄的小枝茎段在离体条件下培养的可行性、再生方式及其形态特征的变化,对木麻黄离体小枝茎段器官的培养及其再生植株的发生与构建进行了研究。结果表明:半木质化的小枝适宜培养,为较好的外植体材料。适宜的继代培养基为改良MS+6BA0.25+NAA0.1+糖30g·L^(−1)+卡拉胶5.5g·L^(−1),不定根诱导培养基为1/2MS+NAA0.5+糖20g·L^(−1)。In order to explore the feasibility of in vitro culture of Casuarinaequisetifolia,the regeneration mode,morphological characteristics,shoot proliferation and development of in vitro propagated plants were studied.The results showed that the semi-lignified twigs were suitable for culture and lead to better quality explants.The optimal subculture medium was found to be modified MS+6BA0.25+NAA0.1+sugar 30 g·L^(−1)+carrageenan 5.5 g·L^(−1),and the best adventitious root induction medium was found to be 1/2MS+NAA0.5+sugar 20 g·L^(−1).

关 键 词：木麻黄 组织培养 植株再生 

分 类 号：S792.93[农业科学—林木遗传育种] S722.37[农业科学—林学]