2型细胞因子白细胞介素4和白细胞介素13对慢性鼻窦炎伴鼻息肉中鼻黏膜上皮细胞自噬的影响  

The effects of type 2 cytokines IL-4 and IL-13 on autophagy of nasal epithelial cells in chronic rhinosinusitis with nasal polyps

作　　者：赵妍 王月 矫健[1,2] 王向东[1,2] 张罗[2,3,4] ZHAO Yan;WANG Yue;JIAO Jian;WANG Xiangdong;ZHANG Luo(Department of Otolaryngology Head and Neck Surgery,Beijing Tongren Hospital,Capital Medical University,Key Laboratory of Otolaryngology Headand Neck Surgery(Capital Medical Medical University),Ministryof Education,Beijing,100730,China;Bejing Institute of Otolaryngology,Bejing KeyLaboratory of Nasal diseases,Beijing,100005,China;Department of Allergy,Capital Medical University,Bejing,100730,China;Research Unit of Diagnosis and Treatment of Chronic Nasal Diseases,Chinese Academyof Medical Sciences,Bejing,10005,China)

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科耳鼻咽喉头颈科学教育部重点实验室(首都医科大学),北京100730 [2]北京市耳鼻咽喉科研究所,鼻病研究北京市重点实验室,北京100005 [3]首都医科大学附属北京同仁医院鼻过敏科,北京100730 [4]中国医学科学院慢性鼻病诊疗策略研究创新单元,北京100005

出　　处：《中国耳鼻咽喉头颈外科》2022年第8期511-515,共5页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金(82000962、81970852、82171110);教育部长江学者创新团队(IRT13082);中国医学科学院医学与健康科技创新工程项目(2019-I2M-5-022);北京市科技计划首都健康保障培育研究项目(Z181100001618002)。

摘　　要：目的 探究2型细胞因子白细胞介素4(interleukin-4,IL-4)和IL-13对慢性鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNP)中鼻黏膜上皮细胞自噬的影响。方法 收集16例CRSwNP息肉组织(其中嗜酸细胞型和非嗜酸细胞型鼻息肉组织各8例)和8例对照黏膜组织。Western blot检测自噬标志蛋白——人微管相关蛋白1A/1B轻链3B(LC3B)的表达,免疫组化染色检测LC3B的表达和分布,免疫荧光染色实验检测LC3B的点状分布。结果 与对照黏膜组织相比,LC3BⅡ的表达在CRSwNP的息肉组织中增加,并且LC3B在嗜酸细胞型鼻息肉上皮层分布最多。IL-4和IL-13可诱导鼻黏膜上皮细胞中LC3BⅡ的表达和促进LC3B的点状分布。结论 与对照黏膜和非嗜酸细胞型鼻息肉组织相比,嗜酸细胞型鼻息肉组织上皮细胞中自噬体数量显著增加。IL-4和IL-13可有效诱导鼻黏膜上皮细胞自噬体增加。本研究为寻找以自噬为靶点的CRSwNP治疗新方法提供理论依据。OBJECTIVE To explore the effect of type 2 cytokines IL-4 and IL-13 on autophagy of nasal epithelial cells in chronic rhinosinusitis with nasal polyps(CRSwNP).METHODSWe collected nasal polyp tissue from 16 patients with CRSwNP(8 eosinophilic nasal polyp and 8 non-eosinophilic nasal polyp) and normal mucosa tissue from 8 healthy control.Western blot was used to detect the expression of autophagy marker protein LC3B.Immunohistochemical staining was used to detect the expression and distribution of LC3B.Immunofluorescence staining was used to detect the LC3B puncta.RESULTS The expression of LC3BⅡ was increased in polyp tissue of CRSwNP compared with control mucosal tissue,and LC3B was most distributed in the nasal epithelial cells of eosinophilic nasal polyps.IL-4 and IL-13 could induce the expression of LC3BⅡ and LC3B puncta in human nasal epithelial cells(HNEPCs).CONCLUSION The number of autophagosomes was significantly increased in the epithelial cells of eosinophilic nasal polyp tissues compared with control mucosa and non-eosinophilic nasal polyp tissues.IL-4 and IL-13 could effectively increase the number of autophagosomes in HNEPCs.Our study will provide a theoretical basis for discovering a novel autophagy-targeting therapeutic method for CRSwNP treatment.

关 键 词：鼻窦炎 鼻息肉 自噬 上皮细胞 细胞因子类 鼻黏膜 2型细胞因子 

分 类 号：R765.41[医药卫生—耳鼻咽喉科] R765.25[医药卫生—临床医学]