硅钨酸盐-双氢氧化物复合材料选择性分离人全血中血红蛋白  

Selective Adsorption of Hemoglobin in Human Whole Blood with Silicotungstate-Layered Double Hydroxides Composite

作　　者：陈晴 王蒙蒙 张杨 张丹丹 CHEN Qing;WANG Meng-meng;ZHANG Yang;ZHANG Dan-dan(Department of Sanitary Inspection,School of Public Health,Shenyang Medical College,Shenyang 110034,China)

机构地区：[1]沈阳医学院公共卫生学院卫生化学教研室,辽宁沈阳110034

出　　处：《化学试剂》2022年第10期1445-1450,共6页Chemical Reagents

基　　金：国家自然科学基金项目(21804093);辽宁省教育厅科学研究项目(SYYX202005);辽宁省博士科研启动基金计划项目(2021-BS-284);沈阳医学院博士科研启动基金项目(20195066)。

摘　　要：在中性条件下,利用静电和氢键相互作用,制备了多金属氧酸盐-双氢氧化物复合材料ZnAl-SiW_(10)Zr,并采用扫描电镜(SEM)、能谱(EDS)、傅里叶变换红外光谱(FT-IR)、X-射线衍射(XRD)手段对ZnAl-SiW_(10)Zr复合材料进行表征。复合材料ZnAl-SiW_(10)Zr中的金属离子与血红蛋白(Hb)之间存在亲和相互作用,因此ZnAl-SiW_(10)Zr可以高选择性吸附Hb。ZnAl-SiW_(10)Zr复合材料(0.5 mg)对1 mL(100μg/mL)血红蛋白的吸附效率在pH 7.0时达到最大,为92%。血红蛋白在材料表面的吸附行为符合Langmuir模型,最大吸附容量为760 mg/g。采用0.1 mol/L SDS溶液作为洗脱剂将固相材料表面吸附的血红蛋白洗脱下来,洗脱回收率为91%。将复合材料ZnAl-SiW_(10)Zr用于实际样品中血红蛋白的分离纯化,并采用SDS-PAGE凝胶电泳验证血红蛋白的纯度,结果表明经复合材料ZnAl-SiW_(10)Zr分离出来的血红蛋白纯度较高。将多金属氧酸盐-双氢氧化物复合材料用于蛋白质的吸附分离,拓宽了多金属氧酸盐在生物分离中的应用。A compositie of silicotungstate-layered double hydroxides ZnAl-SiW_(10)Zr was prepared by using electrostatic and hydrogen bonding interactions at neutral conditions.The ZnAl-SiW_(10)Zr composite was characterized by scanning electron microscopy(SEM),energy-dispersive spectra(EDS),fourier transform infrared spectroscopy(FT-IR)and X-ray diffraction(XRD).On the basis of the affinity interaction between metal ion of ZnAl-SiW_(10)Zr composite and hemoglobin(Hb),the ZnAl-SiW_(10)Zr composite could adsorb Hb selectivity.At pH 7.0,0.5 mg ZnAl-SiW_(10)Zr resulted in the adsorption efficiency of 92%for 1 mL(100μg/mL)hemoglobin.The adsorption behavior of Hb on the composite fitted the Langmuir model with an adsorption capacity of 760 mg/g.The adsorbed Hb could be eluted by the SDS solution(0.1 mol/L)providing recoveries of 91%.The ZnAl-SiW_(10)Zr composite has been used for selectivity separating Hb from human whole blood,and the results of SDS-polyacrylamide gel electrophoresis assay indicated that the Hb with high purity was obtained.It could be expanded the application of polyoxometalates in bioseparation attributing to the application of polyoxometalates composite in protein separation and purification.

关 键 词：血红蛋白 固相萃取 硅钨酸盐 复合材料 双氢氧化物 

分 类 号：O65[理学—分析化学]