A Novel Method for the Quantitative Detection of Sericin Content in Silk Fiber Based on the Ratio of Aspartate to Alanine  

作　　者：Yanfei Feng Jiaming Lin Longxing Niu Peng Pan Xueping Liu Linling Huang Ying Guo Mingzhong Li 

机构地区：[1]National Engineering Laboratory for Modern Silk,College of Textile and Clothing Engineering,Soochow University,Suzhou,215123,China

出　　处：《Journal of Renewable Materials》2023年第1期167-184,共18页可再生材料杂志（英文）

基　　金：supported by the National Key Research and Development Program of China(2017YFC1103602).

摘　　要：The development of a method for the quantitative determination of the sericin content ratio(SCR)is urgently needed for silk refining and the purification of silk fibroin for biomedical applications.In this work,a series of sericin/fibroin mixed samples with known SCRs were prepared by mixing initial samples of extracted sericin and fibroin from Bombyx mori silk.Significant differences were found in the contents of characteristic hydrophilic amino acids abundant in sericin and hydrophobic amino acids abundant in fibroin,and several linear relation-ships of SCR associated with the content ratios of Ser/Ala,Asp/Ala,Lys/Ala,Asp/Gly and Ser/Gly were estab-lished by amino acid analysis.Subsequently,the linear equation expressing SCR(%)as a function of the Asp to Ala content ratio X(%)was established as SCR=2.5634X−12.5587(R 2=0.9972).The results indicated that the SCR of degummed silks calculated by the equation is more objective and effective than the results obtained by the traditional weight loss method.Our study provides a novel approach for the sensitive and quantitative detection of the sericin content within the detection limit in unknown silks,which can contribute to quality control in the silk production process.

关 键 词：SILK sericin content amino acid analysis 

分 类 号：TB33[一般工业技术—材料科学与工程]