葛根芩连汤加减对结直肠癌患者炎症因子和免疫细胞水平影响的临床研究  

Effect of modified Gegen Qinlian Decoction on inflammatory factors and immune cells in patients with colorectal cancer

作　　者：姚忠强[1] 王斐斐 李炜[1] 孙琳 曲凯[2] 白昌儒 贺礼琴 王娟毅[1] YAO Zhongqiang;WANG Feifei;LI Wei;SUN Lin;QU Kai;BAI Changru;HE Liqin;WANG Juanyi(Department of Oncology,No.3201 Hospital Afiliated to Xi'an Jiaotong University,Hanzhong,Shaanxi 723000,China;Department of Hepatobiliary Surgery,The First Affliated Hospital of Xi'an Jiaotong University,Xi'an,Shaanxi 710061,China)

机构地区：[1]西安交通大学附属三二〇一医院肿瘤内科,陕西汉中723000 [2]西安交通大学第一附属医院肝胆外科,陕西西安710061

出　　处：《上海中医药杂志》2022年第7期48-52,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：陕西省科技厅自然科学基础计划研究项目(2017JM8039)。

摘　　要：目的观察葛根芩连汤加减对结直肠癌患者炎症因子和免疫细胞水平的影响。方法将82例拟行手术治疗的结直肠癌患者随机分为对照组和治疗组,每组41例,对照组予常规治疗和限期手术,治疗组在此基础上于手术前7 d开始服用葛根芩连汤加减方。比较两组血浆免疫细胞[CD4^(+)T细胞、CD8^(+)T细胞、自然杀伤(NK)细胞、自然杀伤T(NKT)细胞、调节性T(Treg)细胞]和炎症因子[肿瘤坏死因子-α(TNF-α)、γ干扰素(IFN-γ)、白介素-2(IL-2)、白介素-6(IL-6)、白介素-10(IL-10)、5-羟色胺(5-HT)]水平,以及肿瘤组织中5-HT、TNF-α表达的变化情况。结果①治疗前后组内比较,治疗组血浆CD4^(+)T细胞、NKT细胞水平升高(P<0.05),对照组血浆CD4^(+)T细胞、NKT细胞、CD8^(+)T细胞、NK细胞、Treg细胞水平差异均无统计学意义(P>0.05);组间治疗后比较,治疗组CD4^(+)T细胞、NKT细胞水平高于对照组(P<0.05)。②治疗前后组内比较,治疗组血浆TNF-α、IFN-γ、IL-2、IL-6、IL-10、5-HT水平降低(P<0.05),对照组上述炎症因子水平差异均无统计学意义(P>0.05);组间治疗后比较,治疗组血浆TNF-α、IFN-γ、IL-2、IL-6、IL-10、5-HT水平低于对照组(P<0.05)。③术后与治疗前组内比较,治疗组肿瘤组织中TNF-α、5-HT的累积光密度(IOD)值降低(P<0.05),对照组肿瘤组织中TNF-α、5-HT的IOD值差异无统计学意义(P>0.05);组间术后比较,治疗组肿瘤组织中TNF-α、5-HT的IOD值低于对照组(P<0.05)。结论葛根芩连汤加减治疗可有效调节结直肠癌患者的免疫水平和炎症反应,具有良好的临床应用前景。ObjectiveTo investigate the effect of Modified Gegen Qinlian Decoction(MGQD)on inflammatory factors and immune cells in patients with colorectal cancer.MethodsA total of 82 patients with colorectal cancer to be treated by operation were randomly divided into a control group(n=41)and a treatment group(n=41). Both groups were administered with conventional treatment and confine operation,and the treatment group was additionally administered with MGQD 7 days before operation. The changes of immune cells[CD4^(+)T cells,CD8^(+)T cells,natural killer(NK)cells,natural killer T(NKT)cells,and Treg cells]and inflammatory factors[tumor necrosis factor-α(TNF-α),interferon-gamma(IFN-γ),interleukin-2(IL-2),interleukin-6(IL-6),interleukin-10(IL-10),and 5-hydroxytryptamine(5-HT)]levels in plasma,as well as the changes of 5-HT and TNF-α expression in tumor tissues were compared between the two groups.Results①After treatment,the plasma levels of CD4^(+)T cells and NKT cells in the treatment group increased(P<0.05),but there was no significant difference in the plasma levels of CD4^(+)T cells,NKT cells,CD8^(+)T cells,NK cells and Treg cells before and after treatment in the control group(P>0.05);After treatment,the levels of CD4^(+)T cells and NKT cells in the treatment group were higher than those in the control group(P<0.05). ② According to the intra-group comparison before and after treatment,the plasma levels of TNF-α,IFN-γ,IL-2,IL-6,IL-10,and 5-HT decreased in the treatment group(P<0.05),but the differences in the levels of the above inflammatory factors in the control group were not statistically significant(P>0.05);According to the inter-group comparison after treatment,the plasma levels of TNF-α,IFN-γ,IL-2,IL-6,IL-10,and 5-HT in the treatment group were lower than those in the control group(P<0.05). ③The intra-group comparison before and after treatment showed that the IOD values of TNF-α and 5-HT in the tumor tissues of the treatment group decreased(P<0.05),but there was no significant difference in IOD valu

关 键 词：结直肠癌 葛根芩连汤 免疫调节 炎症因子 中医药疗法 临床试验 

分 类 号：R273[医药卫生—中西医结合]